Characterization of the surface hydrophobicity of filamentous fungi

Authors

  • Theo H. M. Smits,

    Corresponding author
    1. ENAC – ISTE – LPE, Swiss Federal Institute of Technology Lausanne (EPFL), Switzerland.
      *For correspondence at Laboratoire de Biotechnologie Environne-mentale, Bâtiment de Chimie, F1625, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. E-mail theo.smits@epfl.ch; Tel. (+41) 21 693 6231. Fax (+41) 21 693 4722.
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    • Present address: ENAC – ISTE – LBE, Swiss Federal Institute of Technology Lausanne (EPFL), Switzerland.

  • Lukas Y. Wick,

    1. ENAC – ISTE – LPE, Swiss Federal Institute of Technology Lausanne (EPFL), Switzerland.
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  • Hauke Harms,

    1. ENAC – ISTE – LPE, Swiss Federal Institute of Technology Lausanne (EPFL), Switzerland.
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  • Christoph Keel

    1. Institut de Microbiologie Fondamentale, University of Lausanne, CH-1015 Lausanne, Switzerland.
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*For correspondence at Laboratoire de Biotechnologie Environne-mentale, Bâtiment de Chimie, F1625, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. E-mail theo.smits@epfl.ch; Tel. (+41) 21 693 6231. Fax (+41) 21 693 4722.

Summary

A method for the quantitative analysis of the hydrophobicity of the mycelial mat of filamentous fungi based on contact angle measurements is presented. It was tested for a range of fungi belonging to the classes of basidiomycetes, ascomycetes and deuteromycetes. The measured contact angles of the mycelial mats ranged between hydrophilic (<30°) for the deuteromycetes Fusarium oxysporum Fo47 GUS1 and Trichoderma harzianum P1[pZEGA1] and hydrophobic (>60°) for the ascomycete Cladosporium sp. DSE48.1b and the basidiomycetes Paxillus involutus WSL 37.7, Hebeloma crustiliniforme WSL 6.2, Suillus bovinus WSL 48.1 and Laccaria bicolor WSL 73.1. For some fungi, variations in the hydrophobicity of the mycelium depending on the growth medium, the physiological state and the exposure to water were distinguished.

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