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Fig. S1. E. coli containing icsA make actin tails in the cytosol of HeLa cells. HeLa cells were infected with bacteria for 5 h, fixed, and stained with rhodamine phalloidin to show actin filaments. The first column is the fluorescence image, the second column the phase contrast image, and the third column is the overlay of the fluorescence and phase contrast images. Each row represents a different strain: S. flexneri 2457T (A-C); S. flexneri BS520 (D-F); DMM1 (G-I) the arrows point to bacteria with actin polymerized at the surface; DMM2 (J-L) arrow heads point out membrane protrusions and the star is next to a bacterium with an actin ?comet tail? along the side; DMM3 (M-O); DMM4 (P-R); DMM5 (S-U) arrow heads point out membrane protrusions. The bar is 2 mm in length. Fig. S1 continued. E. coli containing icsA make actin tails in the cytosol of HeLa cells. HeLa cells were infected with bacteria for 5 h, fixed, and stained with rhodamine phalloidin to show actin filaments. The first column is the fluorescence image, the second column the phase contrast image, and the third column is the overlay of the fluorescence and phase contrast images. Each row represents a different strain: S. flexneri 2457T (A-C); S. flexneri BS520 (D-F); DMM1 (G-I) the arrows point to bacteria with actin polymerized at the surface; DMM2 (J-L) arrow heads point out membrane protrusions and the star is next to a bacterium with an actin ?comet tail? along the side; DMM3 (M-O); DMM4 (P-R); DMM5 (S-U) arrow heads point out membrane protrusions. The bar is 2 mm in length.

FilenameFormatSizeDescription
CMI_143_sm_fsm1.AVI12814KSupporting info item
CMI_143_sm_fsm2.AVI52231KSupporting info item
CMI_143_sm_fsms1.gif215KSupporting info item
CMI_143_sm_fsms2.gif232KSupporting info item

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