Muscle precursor cell autografting in a murine model of urethral sphincter injury
Article first published online: 30 JAN 2002
Volume 89, Issue 3, pages 298–302, February 2002
How to Cite
Yiou, R., Dreyfus, P., Chopin, D.K., Abbou, C.-C. and Lefaucheur, J.-P. (2002), Muscle precursor cell autografting in a murine model of urethral sphincter injury. BJU International, 89: 298–302. doi: 10.1046/j.1464-4096.2001.01618.x
- Issue published online: 30 JAN 2002
- Article first published online: 30 JAN 2002
- Accepted for publication 28 August 2001
- muscle regeneration;
- muscle precursor cells;
- urethral sphincter insufficiency
Objective To determine whether muscle precursor cells (MPCs) harvested from limb skeletal muscle can enhance the regeneration process of the striated urethral sphincter after injury.
Material and methods Striated urethral sphincters of male mice were injured by an injection of a myotoxic substance (notexin). In the experimental group, 2 days after injury, MPCs were enzymatically harvested from striated muscles of the lower limbs and labelled with PKH 26, then immediately re-injected into the injured urethral sphincter of the same animal. In the control group, saline buffer was injected instead of MPCs. Animals were killed 7 days or 1 month after injury and the sphincters removed for histological study (the presence of PKH 26-labelled myofibres, measurement of myofibre diameter and total number of myofibres).
Results MPC autografting accelerated sphincter muscle repair, as shown by a higher myofibre diameter (P = 0.03) and number (P = 0.01) in the experimental group than in the controls at 7 days. One month after their injection MPCs were still detectable in the regenerating sphincters and participated in the formation of new myofibres.
Conclusion This study provides the experimental basis for a new therapeutic approach to urethral sphincter insufficiency after surgical or obstetrical injury, based on MPC autografting.