A sequence mutation in the cinnamyl alcohol dehydrogenase gene associated with altered lignification in loblolly pine
Article first published online: 18 JUL 2003
Plant Biotechnology Journal
Volume 1, Issue 4, pages 253–258, July 2003
How to Cite
Gill, G. P., Brown, G. R. and Neale, D. B. (2003), A sequence mutation in the cinnamyl alcohol dehydrogenase gene associated with altered lignification in loblolly pine. Plant Biotechnology Journal, 1: 253–258. doi: 10.1046/j.1467-7652.2003.00024.x
- Issue published online: 18 JUL 2003
- Article first published online: 18 JUL 2003
- Received 16 December 2002; revised 14 February 2003; accepted 25 February 2003.
Evidence for the molecular basis of a null allele of cinnamyl alcohol dehydrogenase (CAD) has been discovered in the loblolly pine (Pinus taeda L.) clone 7-56. The mutation is a two-base pair adenosine insertion located in exon 5 that causes a frame-shift which is predicted to result in premature termination of the protein. For routine detection of the mutation, a diagnostic assay was developed utilizing Template-directed Dye-terminator Incorporation and Fluorescence Polarization detection (FP-TDI). Loblolly pine is the most important commercial tree species in the USA, being harvested for pulp and solid wood products. Chemical pulping could be increased in efficiency by selecting for trees having a two-base pair adenosine insertion, by use of the rapid diagnostic assay developed in this study.