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Porins and lipopolysaccharide from Salmonella typhimurium regulate the expression of CD80 and CD86 molecules on B cells and macrophages but not CD28 and CD152 on T cells

  1. M. Galdiero1,
  2. M. G. Pisciotta2,
  3. E. Galdiero3,
  4. C. Romano Carratelli1

Article first published online: 31 OCT 2003

DOI: 10.1046/j.1469-0691.2003.00728.x

Issue

Clinical Microbiology and Infection

Clinical Microbiology and Infection

Volume 9, Issue 11, pages 1104–1111, November 2003

Additional Information

How to Cite

Galdiero, M., Pisciotta, M. G., Galdiero, E. and Carratelli, C. R. (2003), Porins and lipopolysaccharide from Salmonella typhimurium regulate the expression of CD80 and CD86 molecules on B cells and macrophages but not CD28 and CD152 on T cells. Clinical Microbiology and Infection, 9: 1104–1111. doi: 10.1046/j.1469-0691.2003.00728.x

Author Information

  1. 1

    Dipartimento di Medicina Sperimentale, Facoltà di Medicina e Chirurgia, Seconda Università di Napoli,

  2. 2

    Dipartimento di Patologia Generale, Facoltà di Medicina e Chirurgia, Seconda Università di Napoli, and

  3. 3

    Dipartimento di Fisiologia Generale, Sezione di Igiene e Microbiologia, Università degli Studi di Napoli ‘Federico II’, Napoli, Italy

*Corresponding author and reprint requests: Caterina Romano Carratelli, Dipartimento di Medicina Sperimentale, Facoltà di Medicina e Chirurgia, Seconda Università di Napoli.Via Costantinopoli, 16–80138 Napoli, Italy Tel: +39 81 5665658 Fax: +39 81 5667578 E-mail: caterina.romano@unina2.it

Publication History

  1. Issue published online: 31 OCT 2003
  2. Article first published online: 31 OCT 2003
  3. Accepted 10 January 2002

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Objective  The aim of this study was to evaluate the effect of porins from Salmonella typhimurium on costimulatory molecules such as CD80/CD86 and CD28/CD152. The interactions between these molecules are able to influence the immune response through the regulation of cytokines release which, on their own, are able to regulate the immunological response by a feedback mechanism.

Methods S. typhimurium strain SH5014 (a rough lipopolysaccharide (LPS) producing strain) was used as the source of porins and LPS. Peripheral blood mononuclear cells were obtained from healthy adult donors. THP1 cells were obtained from ATCC (Rockville, MD, USA). Immunofluorescence and flow cytometry were performed using a FACS IV (Becton–Dickinson, Mountain View, CA, USA).

Results  Our results show that porins of S. typhimurium increase the expression of CD86 and the expression of CD80 both on B lymphocytes and macrophages, while the expression of CD28 and CD152 on T lymphocytes was unaltered. The expression of CD80 and CD86 is dose-dependent and starts after 24 h post treatment, peaks at 48 h and goes back to the basal value after 72 h.

Conclusions S. typhimurium porins are able to induce a high expression of costimulatory molecules (CD80 and CD86) on lymphocytes and macrophages.

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