Transabdominal first trimester embryofetoscopy as a potential approach to early in utero stem cell transplantation and gene therapy
Article first published online: 23 DEC 2002
Copyright © 2000 International Society of Ultrasound in Obstetrics and Gynecology
Ultrasound in Obstetrics & Gynecology
Volume 15, Issue 4, pages 302–307, April 2000
How to Cite
Surbek, D.V., Tercanli, S. and Holzgreve, W. (2000), Transabdominal first trimester embryofetoscopy as a potential approach to early in utero stem cell transplantation and gene therapy. Ultrasound Obstet Gynecol, 15: 302–307. doi: 10.1046/j.1469-0705.2000.00089.x
- Issue published online: 23 DEC 2002
- Article first published online: 23 DEC 2002
- Manuscript Accepted: 25 FEB 2000
- Manuscript Revised: 4 OCT 1999
- Manuscript Received: 16 FEB 1999
- Cited By
- in utero stem cell therapy;
- fetal gene therapy
To explore the potential of embryofetoscopy for early diagnosis and for access to the fetal circulation in the first trimester of gestation.
Transabdominal embryofetoscopy was performed in 14 patients scheduled for termination of pregnancy using a 1-mm semirigid fibreoptic telescope with a 18 gauge examination sheath and a single-chip digital camera. A 25 gauge needle was inserted through an additional 21 gauge side port to access the fetal circulation.
Fetal head, face, abdomen, complete upper and lower limbs could be visualized in over 80% of cases. On the contrary, the fetal back and external genitalia could be examined in detail only in some cases (35.7% and 64.3%, respectively). Injection of 10–20 ml saline improved visibility in 43% of cases. Funipuncture was successful in two of three attempts.
Our experience suggests that embryofetoscopy is a useful tool for early diagnosis in the first trimester of pregnancy. Funipuncture is possible thus providing the means for an early intravascular stem cell application. Copyright © 2000 International Society of Ultrasound in Obstetrics and Gynecology