From a paper presented at the International Symposium on Embryonic Stem Cells – Prospects for Human Health, at the University of Sheffield, UK, 10 September 2001.
Surface antigens of human embryonic stem cells: changes upon differentiation in culture*
Article first published online: 25 APR 2002
Journal of Anatomy
Volume 200, Issue 3, pages 249–258, March 2002
How to Cite
Draper, J. S., Pigott, C., Thomson, J. A. and Andrews, P. W. (2002), Surface antigens of human embryonic stem cells: changes upon differentiation in culture. Journal of Anatomy, 200: 249–258. doi: 10.1046/j.1469-7580.2002.00030.x
- Issue published online: 25 APR 2002
- Article first published online: 25 APR 2002
- Accepted for publication 18 January 2002
- stem cells;
- surface antigens
We have analysed the surface antigen phenotype of a human embryonic stem (hES) cell line (H7) and the changes that occur upon differentiation induced by retinoic acid, hexamethylene bisacetamide and dimethylsulphoxide. The undifferentiated stem cells expressed Stage Specific Embryonic Antigen-3 (SSEA3), SSEA4, TRA-1-60, and TRA-1-8 but not SSEA1. In these characteristics they closely resemble human embryonal carcinoma (EC) cells derived from testicular teratocarcinomas, and are distinct from murine EC and ES cells. The undifferentiated cells also expressed the liver/bone/kidney isozyme of alkaline phosphatase detected by antibody TRA-2-54, the class 1 major histocompatability antigens, HLA-ABC, and the human Thy1 antigen. Differentiation of hES cells was induced by retinoic acid, HMBA and DMSO with the appearance of various cell types including neurons and muscle cells. The surface antigens characteristically expressed by hES cells were down-regulated following induction of differentiation and other antigens appeared, notably several ganglioside glycolipids detected by antibodies VIN-IS-56 (GD3 and GD2), VIN-2PB-22 (GD2), A2B5 (GT3) and ME311 (9-O-acetyl-GD3). Whereas the expression of HLA was slightly down-regulated upon differentiation, its expression was strongly induced by interferon-γ in both the undifferentiated and the differentiated cells, although the induction in the differentiated cultures was considerably stronger than in the stem cells. In all of these features the human ES cells, and their pattern of differentiation, resembled the pluripotent human EC cell line NTERA-2 although clearly the range of cells generated by the hES cells was considerably greater.