From a paper presented at the International Symposium on Embryonic Stem Cells – Prospects for Human Health, at the University of Sheffield, UK, 10 September 2001.
Derivation and spontaneous differentiation of human embryonic stem cells*
Article first published online: 25 APR 2002
Journal of Anatomy
Volume 200, Issue 3, pages 225–232, March 2002
How to Cite
Amit, M. and Itskovitz-Eldor, J. (2002), Derivation and spontaneous differentiation of human embryonic stem cells. Journal of Anatomy, 200: 225–232. doi: 10.1046/j.1469-7580.2002.00032.x
- Issue published online: 25 APR 2002
- Article first published online: 25 APR 2002
- Accepted for publication 5 February 2002
- cell lines;
- embryo; embryoid bodies;
- stem cell technology
Embryonic stem (ES) cells are unique cells derived from the inner cell mass of the mammalian blastocyst. These cells are immortal and pluripotent, retain their developmental potential after prolonged culture, and can be continuously cultured in an undifferentiated state. Many in vitro differentiation systems have been developed for mouse ES cells, including reproducible methods for mouse ES cell differentiation into haematopoietic and neural precursors, cardiomyocytes, insulin-secreting cells, endothelial cells and various other cell types. The derivation of new human ES cell lines provides the opportunity to develop unique models for developmental research and for cell therapies. In this review we consider the derivation and spontaneous differentiation of human ES cells.