Involvement of endogenous salicylic acid content, lipoxygenase and antioxidant enzyme activities in the response of tomato cell suspension cultures to NaCl

Authors

  • Anabel Molina,

    1. Departamento de Bioquímica, Biología Celular y Molecular de Plantas. Estación Experimental del Zaidín, CSIC, Apartado 419, 18008-Granada, Spain
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  • Pablo Bueno,

    1. Departamento de Bioquímica, Biología Celular y Molecular de Plantas. Estación Experimental del Zaidín, CSIC, Apartado 419, 18008-Granada, Spain
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  • María Carmen Marín,

    1. Departamento de Bioquímica, Biología Celular y Molecular de Plantas. Estación Experimental del Zaidín, CSIC, Apartado 419, 18008-Granada, Spain
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  • María Pilar Rodríguez-Rosales,

    1. Departamento de Bioquímica, Biología Celular y Molecular de Plantas. Estación Experimental del Zaidín, CSIC, Apartado 419, 18008-Granada, Spain
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  • Andrés Belver,

    1. Departamento de Bioquímica, Biología Celular y Molecular de Plantas. Estación Experimental del Zaidín, CSIC, Apartado 419, 18008-Granada, Spain
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  • Kees Venema,

    1. Departamento de Bioquímica, Biología Celular y Molecular de Plantas. Estación Experimental del Zaidín, CSIC, Apartado 419, 18008-Granada, Spain
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  • Juan Pedro Donaire

    Corresponding author
    1. Departamento de Bioquímica, Biología Celular y Molecular de Plantas. Estación Experimental del Zaidín, CSIC, Apartado 419, 18008-Granada, Spain
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Author for correspondence: Juan Pedro Donaire Tel: +34 958 121011 Fax: +34 958 129600 Email: juanpedro.donaire@eez.csic.es

Summary

  • •   The effects of salt stress and adaptation on salicylic acid (SA) content and on antioxidant and lipoxygenase (LOX) enzyme activities were studied in tomato (Lycopersicon esculentum cv. Pera) cells.
  • •   NaCl-adapted cells were obtained from calli adapted to 100 mm NaCl by successive subcultures in medium supplemented with 100 mm NaCl. Salt stress treatments consisted of the addition of 100 mm NaCl to cells.
  • •   Adapted cells contained a lower concentration of SA than unadapted cells. The lower manganese-containing superoxide dismutase (Mn-SOD) and LOX activities as well as the higher glutathione reductase (GR) and ascorbate peroxidase (APX) activities in adapted cells than in unadapted cells could be correlated with the development of salt adaptation. Salt stress increased APX and LOX activities as well as lipid peroxidation in unadapted cells and increased Mn-SOD activity in both types of cells. Application of 200 µm SA + 100 mm NaCl inhibited APX activity in both unadapted and adapted cells, induced the Mn-SOD in adapted cells and increased lipid peroxidation in unadapted cells.
  • •   Our data indicate that adaptation of tomato cells to NaCl results in a higher tolerance to NaCl-induced oxidative stress and suggest a role for SA in this response.

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