Targeted proteomics to identify cadmium-induced protein modifications in Glomus mosseae-inoculated pea roots

Authors

  • Ombretta Repetto,

    Corresponding author
    1. UMR 1088 BBCE-IPM, INRA-CMSE, BP 86510, 21065 Dijon Cedex, France;
    2. Università del Piemonte Orientale ‘Amedeo Avogadro’, Dipartimento di Scienze e Tecnologie Avanzate, C.so Borsalino, 54–15100, Alessandria, Italy
      Author for correspondence: Ombretta Repetto Tel: +39 131 283840 Fax: +39 131 254410 Email: orepetto@unipmn.it
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  • Gwénäelle Bestel-Corre,

    1. UMR 1088 BBCE-IPM, INRA-CMSE, BP 86510, 21065 Dijon Cedex, France;
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  • Eliane Dumas-Gaudot,

    1. UMR 1088 BBCE-IPM, INRA-CMSE, BP 86510, 21065 Dijon Cedex, France;
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  • Graziella Berta,

    1. Università del Piemonte Orientale ‘Amedeo Avogadro’, Dipartimento di Scienze e Tecnologie Avanzate, C.so Borsalino, 54–15100, Alessandria, Italy
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  • Vivienne Gianinazzi-Pearson,

    1. UMR 1088 BBCE-IPM, INRA-CMSE, BP 86510, 21065 Dijon Cedex, France;
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  • Silvio Gianinazzi

    1. UMR 1088 BBCE-IPM, INRA-CMSE, BP 86510, 21065 Dijon Cedex, France;
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Author for correspondence: Ombretta Repetto Tel: +39 131 283840 Fax: +39 131 254410 Email: orepetto@unipmn.it

Summary

  • •   Arbuscular mycorrhiza (AM) can increase plant tolerance to heavy metals. A targeted proteomic approach was used to determine the putative identity of some of the proteins induced/modulated by cadmium (Cd) and to analyse the impact of the mycorrhizal process.
  • •   The effect of Cd (100 mg Cd kg-1 substrate) applied either at planting or 15 d later on two pea (Pisum sativum) genotypes, differing in sensitivity to Cd inoculated or not with the AM fungus Glomus mosseae, was studied at three levels: plant biomass production, development of G. mosseae and root differential protein display with one- and two-dimensional gel electrophoresis (1-DE and 2-DE) analyses.
  • •   Cd-induced growth inhibition was significantly alleviated by mycorrhiza in the Cd-sensitive genotype. The AM symbiosis modulated the expression of several proteins, identified by liquid chromatography–tandem mass spectrometry, newly induced and upregulated or downregulated by Cd.
  • •   The protective effect of AM symbiosis towards Cd stress was observed in the Cd-sensitive genotype. Our results demonstrate the usefulness of proteomics to better understand the possible role of AM symbiosis in detoxification/response mechanisms towards Cd in pea plants.

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