Identification of laccase-like genes in ectomycorrhizal basidiomycetes and transcriptional regulation by nitrogen in Piloderma byssinum

Authors

  • David M. Chen,

    1. Mycorrhiza Research Group, Centre for Horticulture and Plant Sciences, Parramatta Campus, University of Western Sydney, Locked Bag 1797, Penrith South and NSW 1797, Australia;
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  • Brigitte A. Bastias,

    1. Mycorrhiza Research Group, Centre for Horticulture and Plant Sciences, Parramatta Campus, University of Western Sydney, Locked Bag 1797, Penrith South and NSW 1797, Australia;
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  • Andrew F. S. Taylor,

    1. Department of Forest Mycology & Pathology, Swedish University of Agricultural Sciences, Box 7026, S–75007 Uppsala, Sweden
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  • John W. G. Cairney

    Corresponding author
    1. Mycorrhiza Research Group, Centre for Horticulture and Plant Sciences, Parramatta Campus, University of Western Sydney, Locked Bag 1797, Penrith South and NSW 1797, Australia;
      Author for correspondence: John W. G. Cairney Tel: +61 29685 9903 Fax: +61 29685 9915 Email: j.cairney@uws.edu.au
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Author for correspondence: John W. G. Cairney Tel: +61 29685 9903 Fax: +61 29685 9915 Email: j.cairney@uws.edu.au

Summary

  • •   Ectomycorrhizal (ECM) fungi were screened for laccase-like genes by polymerase chain reaction (PCR) using primers for white rot fungal laccase genes, and expression of the genes was examined by reverse transcriptase polymerase chain reaction (RT-PCR) for Piloderma byssinum in axenic culture under different nutrient conditions.
  • •   Laccase-like genes were present in Rhizopogon roseolus along with several Russulales and Atheliaceae taxa, and showed strong nucleotide sequence similarity to laccase genes in white rot fungi. Multiple laccase-like genes were only identified in Piloderma spp.
  • •   Laccase-like genes were expressed in Piloderma spp., with transcript levels some six times higher under high nitrogen conditions in P. byssinum than when nitrogen availability was lower.
  • •   The potential roles of laccases in nutrient mobilization and/or differentiation of multihyphal ECM fungal structures are discussed.

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