Inter- and intraspecific variation of homologous hydrophobin (H1) gene sequences among Xanthoria spp. (lichen-forming ascomycetes)
Author for correspondence: Rosmarie Honegger Tel: +41 1634 8243 Fax: +41 1634 8204 Email: firstname.lastname@example.org
- • Inter- and intra-specific variability of the hydrophobin gene H1 was investigated in a range of Xanthoria species collected in their natural habitat.
- • On Southern blots the XEH1 probe (from Xanthoria ectaneoides) hybridized under standard conditions to the genomic DNA of Xanthoria calcicola, Xanthoria parietina and Xanthoria flammea. The corresponding H1 genes in these and additional species (Xanthoria turbinata, Xanthoria spp.), and ribosomal gene regions (ITS 1 and 2, 5.8S rDNA) were amplified with polymerase chain reaction, sequenced and similarities calculated. Restriction fragment length polymorphism and cleaved amplified polymorphic sequence analyses provided molecular tools for species delimitation.
- • Ribosomal gene data were largely in parallel with the hydrophobin H1 sequence data, the protein-coding H1 sequence being even more variable as the noncoding rDNA regions.
- • Very low intraspecific variation was detected in the H1 gene of X. parietina samples collected on four continents, with the highest variability occurring among Mediterranean samples. Xanthoria ectaneoides from Brittany, France, and X. calcicola appear to be coastal and inland forms of the same fungal species but differ from Sicilian X. ectaneoides. The African X. flammea and X. turbinata are related to the X. parietina cluster.