Analysis of expressed sequence tags from the ectomycorrhizal basidiomycetes Laccaria bicolor and Pisolithus microcarpus

Authors

  • Martina Peter,

    1. Unité Mixte de Recherche INRA–UHP 1136 ‘Interactions Arbres/Microorganismes’, Centre de Recherches de Nancy, 54280 Champenoux, France;
    2. These authors contributed equally to this work
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  • Pierre-Emmanuel Courty,

    1. Unité Mixte de Recherche INRA–UHP 1136 ‘Interactions Arbres/Microorganismes’, Centre de Recherches de Nancy, 54280 Champenoux, France;
    2. These authors contributed equally to this work
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  • Annegret Kohler,

    1. Unité Mixte de Recherche INRA–UHP 1136 ‘Interactions Arbres/Microorganismes’, Centre de Recherches de Nancy, 54280 Champenoux, France;
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  • Christine Delaruelle,

    1. Unité Mixte de Recherche INRA–UHP 1136 ‘Interactions Arbres/Microorganismes’, Centre de Recherches de Nancy, 54280 Champenoux, France;
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  • David Martin,

    1. Unité Mixte de Recherche INRA–UHP 1136 ‘Interactions Arbres/Microorganismes’, Centre de Recherches de Nancy, 54280 Champenoux, France;
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  • Denis Tagu,

    1. INRA Rennes, Unité Mixte de Recherche BiO3P, BP 35327, 35653 Le Rheu Cedex, France;
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  • Pascale Frey-Klett,

    1. Unité Mixte de Recherche INRA–UHP 1136 ‘Interactions Arbres/Microorganismes’, Centre de Recherches de Nancy, 54280 Champenoux, France;
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  • Sébastien Duplessis,

    1. Unité Mixte de Recherche INRA–UHP 1136 ‘Interactions Arbres/Microorganismes’, Centre de Recherches de Nancy, 54280 Champenoux, France;
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  • Michel Chalot,

    1. Unité Mixte de Recherche INRA–UHP 1136 ‘Interactions Arbres/Microorganismes’, Centre de Recherches de Nancy, 54280 Champenoux, France;
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  • Gopi Podila,

    1. Dept. of Biological Sciences, University of Alabama, Huntsville, AL 35899, USA;
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  • Francis Martin

    1. Unité Mixte de Recherche INRA–UHP 1136 ‘Interactions Arbres/Microorganismes’, Centre de Recherches de Nancy, 54280 Champenoux, France;
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Author for correspondence: Martina Peter Tel: +33 383 39 40 80 Fax: +33 383 39 40 69 Email: peter@nancy.inra.fr

Summary

  • • In an effort to discover genes that are expressed in the ectomycorrhizal basidiomycetes Laccaria bicolor and Pisolithus microcarpus, and in P. microcarpus/Eucalyptus globulus ectomycorrhizas, we have sequenced 1519 and 1681 expressed sequence tags (ESTs) from L. bicolor and P. microcarpus cDNA libraries.
  • • Contig analysis resulted in 905 and 806 tentative consensus sequences (unique transcripts) in L. bicolor and P. microcarpus, respectively. For 36% of the ESTs, significant similarities to sequences in databases were detected. The most abundant transcripts showed no similarity to previously identified genes. Sequence redundancy analysis between different developmental stages indicated that several genes were differentially expressed in free-living mycelium and symbiotic tissues of P. microcarpus.
  • • Based on sequence similarity, 11% of L. bicolor unique transcripts were also detected in P. microcarpus. Similarly, L. bicolor and P. microcarpus shared only a low proportion of common transcripts with other basidiomycetous fungi, such as Pleurotus ostreatus and Agaricus bisporus. Such a low proportion of shared transcripts between basidiomycetes suggests, on the one hand, that the variability of expressed transcripts in different fungi and fungal tissues is considerably high. On the other hand, it might reflect the low number of GenBank entries of basidiomycetous origin and stresses the necessity of an additional sequencing effort.
  • • The present ESTs provide a valuable resource for future research on the development and functioning of ectomycorrhizas.

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