Lipid composition of Botrytis cinerea and inhibition of its radiolabelling by the fungicide iprodione
Article first published online: 31 JUL 2003
Volume 160, Issue 1, pages 199–207, October 2003
How to Cite
Griffiths, R. G., Dancer, J., O'Neill, E. and Harwood, J. L. (2003), Lipid composition of Botrytis cinerea and inhibition of its radiolabelling by the fungicide iprodione. New Phytologist, 160: 199–207. doi: 10.1046/j.1469-8137.2003.00848.x
- Issue published online: 31 JUL 2003
- Article first published online: 31 JUL 2003
- Received: 20 March 2003 Accepted: 30 May 2003; doi: 10.1046/j.1469-8137.2003.00848.x
- Botrytis cinerea;
- dicarboximide fungicide;
- • Botrytis cinerea is an important plant pathogen that causes grey mould in over 200 hosts. It is often controlled by dicarboximides, which have various proposed mechanisms of action, including effects on lipids. Here we have examined the effect of one dicarboximide, iprodione, on lipid metabolism.
- • B. cinerea, cultured in malt extract media, was challenged with iprodione and its lipids extracted, separated by TLC, and analysed by GLC. Lipid metabolism was followed using [1-14C]acetate.
- • Triacylglycerol was the major nonpolar and phosphatidylcholine the main polar lipid in B. cinerea. Linoleate, followed by α-linolenate, were the major fatty acids and most lipid classes had compositions broadly similar to the total fatty acid pattern. Iprodione, at concentrations causing a cessation of growth (5 µM) caused a decrease in polar lipid but not total nonpolar lipid labelling. Within the nonpolar lipids, DAG was better labelled.
- • The data show that iprodione had a selective effect on lipid metabolism. The altered pattern of labelling suggested that choline (ethanolamine) phosphotransferase would be worth investigating as a primary site of action.