On the unreliability of published DNA sequences
Article first published online: 14 AUG 2003
Volume 160, Issue 1, pages 43–48, October 2003
How to Cite
Bridge, P. D., Roberts, P. J., Spooner, B. M. and Panchal, G. (2003), On the unreliability of published DNA sequences. New Phytologist, 160: 43–48. doi: 10.1046/j.1469-8137.2003.00861.x
- Issue published online: 14 AUG 2003
- Article first published online: 14 AUG 2003
- Received: 17 March 2003 Accepted: 16 June 2003; doi: 10.1046/j.1469-8137.2003.00861.x
- DNA sequences;
- ribosomal RNA;
- • Here, the reliability of published fungal nucleic acid sequences is tested by the critical re-evaluation of 206 named sequences obtained from public-access databases.
- • Sequences from the ribosomal RNA (rRNA) gene cluster were examined as these are commonly used to establish fungal phylogeny and evolution, and are also increasingly employed in the identification of fungi from nonculture based studies.
- • Fifty-one rRNA internal transcribed spacer (ITS) sequences were obtained for species of Amanita, 55 ITS sequences were obtained for species of Phoma and 100 rRNA small subunit sequences were obtained from representative genera of the order Helotiales. In each case, the fungal group was selected partly on the basis of sequences deposited by three or more laboratories in order to avoid sample bias. The results suggest that up to 20% of the sequences available for each group may be unreliable, and this proportion is supported by additional informal observations.