Dr. Paul Mandel died on October 6, 1992.
Age-Associated Changes of Rat Brain Neuronal and Astroglial Poly(ADP-Ribose) Polymerase Activity
Article first published online: 23 NOV 2002
Journal of Neurochemistry
Volume 62, Issue 2, pages 502–506, February 1994
How to Cite
Messripour, M., Weltin, D., Rastegar, A., Ciesielski, L., Kopp, P., Chabert, M. and Mandel, P. (1994), Age-Associated Changes of Rat Brain Neuronal and Astroglial Poly(ADP-Ribose) Polymerase Activity. Journal of Neurochemistry, 62: 502–506. doi: 10.1046/j.1471-4159.1994.62020502.x
- Issue published online: 23 NOV 2002
- Article first published online: 23 NOV 2002
- Received April 19, 1993; revised manuscript received June 21, 1993; accepted July 6, 1993.
- Poly(ADP-ribose) polymerase;
Abstract: Nuclear poly(ADP-ribose) polymerase levels as well as the DNA strand break levels of whole-brain neuronal and astroglial cells were investigated. Three- and 30-month-old rats were used. Low-molecular-weight neurofilaments and glutamine synthetase served as neuronal and astroglial markers, respectively. A large increase in the poly(ADP-ribose) polymerase activity was observed in the neurons (threefold) and astrocytes (3.7-fold) derived from 30-month-old rats. Similarly, the amount of poly(ADP-ribose) polymerase, evaluated per milligram of DNA, increased ∼3.5-fold in neurons and 3.9-fold in astrocytes prepared from 30-month-old rats. Whether the increase in the poly(ADP-ribose) polymerase activity was due to an enhanced rate of DNA strand break was investigated by determining the rate of DNA unwinding. A significant increase in DNA unwinding rate was detected in the neurons (2.7-fold), although a lower increase was observed in the astroglia (1.3-fold) of aged animals.