Organization, Sequence, and Expression of a Gene Encoding Goldfish Neurofilament Medium Protein

Authors

  • Eric Glasgow,

    1. Department of Psychiatry and Behavioral Science and
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  • Christina M. Hall,

    1. Institute of Neurobiology, Department of Anatomy and Cell Biology, University of Göteborg, Göteborg, Sweden
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  • Nisson Schechter

    Corresponding author
    1. Department of Psychiatry and Behavioral Science and
    2. Department of Biochemistry and Cell Biology, State University of New York at Stony Brook, Stony Brook, New York, U.S.A.; and
      Address correspondence and reprint requests to Dr. N. Schechter at Department of Psychiatry and Behavioral Science, Health Sciences Center, State University of New York, Stony Brook, NY 11794, U.S.A.
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Address correspondence and reprint requests to Dr. N. Schechter at Department of Psychiatry and Behavioral Science, Health Sciences Center, State University of New York, Stony Brook, NY 11794, U.S.A.

Abstract

Abstract: The goldfish visual pathway displays a remarkable capacity for continuous neurogenesis, plasticity, and regeneration. The intermediate filament protein composition of this system differs from that of higher vertebrates, which lack the capacity for continued nerve growth and development. In an effort to determine how intermediate filament proteins are regulated during nerve growth, we isolated and characterized cDNA and genomic clones representing the goldfish neurofilament medium (NF-M) protein. The tissue-specific expression of goldfish NF-M mRNA was analyzed by RNase protection assays and by in situ hybridization. The expression of goldfish NF-M is qualitatively the same as in other species. Although the intermediate filament protein composition of the goldfish visual pathway is unusual when compared with higher vertebrates, the goldfish NF-M protein is similar to higher vertebrate NF-M proteins. In addition, the organization of the goldfish NF-M gene is identical to the NF-M genes in all other vertebrate species. In contrast, the promoter region of the goldfish NF-M gene has several potential regulatory sequences that are not found in the promoter regions of higher vertebrate NF-M genes.

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