Extension of the 2-Deoxyglucose Method to the Fetus in Utero: Theory and Normal Values for the Cerebral Glucose Consumption in Fetal Guinea Pigs
Article first published online: 23 NOV 2002
Journal of Neurochemistry
Volume 63, Issue 1, pages 271–279, July 1994
How to Cite
Berger, R., Gjedde, A., Heck, J., Müller, E., Krieglstein, J. and Jensen, A. (1994), Extension of the 2-Deoxyglucose Method to the Fetus in Utero: Theory and Normal Values for the Cerebral Glucose Consumption in Fetal Guinea Pigs. Journal of Neurochemistry, 63: 271–279. doi: 10.1046/j.1471-4159.1994.63010271.x
- Issue published online: 23 NOV 2002
- Article first published online: 23 NOV 2002
- Received March 15, 1993; final revised manuscript received October 26, 1993; accepted November 22, 1993.
- Cited By
- Cerebral metabolism;
- Fetal guinea pig;
- Lumped constant;
Abstract: Fetal cerebral metabolism changes during development. The normal fetal metabolic rate must be known to evaluate pathophysiological changes. Therefore, we determined the regional cerebral glucose consumption in the fetal guinea pig. This required the application of the 2-deoxyglucose method to this species. We measured both the transfer coefficients of deoxyglucose and glucose between the maternal arterial plasma and the fetal brain and the lumped constant in chronically prepared undisturbed guinea pig dams using a three-compartment model. Furthermore, the ratio between the initial clearances of deoxyglucose and glucose between the maternal arterial plasma and the fetal brain and the ratio between the phosphorylation coefficients of these substrates in the fetal brain were determined. The total cerebral glucose consumption measured by the deoxyglucose method (10 ± 1.2 µmol/100 g/min) was similar to that calculated from the glucose concentration and the phosphorylation coefficient of glucose in the cerebrum (10 ± 0.4 µmol/100 g/min). We conclude that the 2-deoxyglucose method is applicable to the guinea pig, and we further conclude that in the fetal guinea pig cerebral glucose consumption is 10 times lower than that in the adult.