Coordinate Regulation of Choline Acetyltransferase, Tyrosine Hydroxylase, and Neuropeptide mRNAs by Ciliary Neurotrophic Factor and Leukemia Inhibitory Factor in Cultured Sympathetic Neurons
Version of Record online: 23 NOV 2002
Journal of Neurochemistry
Volume 63, Issue 2, pages 429–438, August 1994
How to Cite
Lewis, S. E., Rao, M. S., Symes, A. J., Dauer, W. T., Fink, J. S., Landis, S. C. and Hyman, S. E. (1994), Coordinate Regulation of Choline Acetyltransferase, Tyrosine Hydroxylase, and Neuropeptide mRNAs by Ciliary Neurotrophic Factor and Leukemia Inhibitory Factor in Cultured Sympathetic Neurons. Journal of Neurochemistry, 63: 429–438. doi: 10.1046/j.1471-4159.1994.63020429.x
- Issue online: 23 NOV 2002
- Version of Record online: 23 NOV 2002
- Received September 22, 1993; revised manuscript received December 2, 1993; accepted December 13, 1993.
- Neurotransmitter expression;
- Ciliary neurotrophic factor;
- Cholinergic differentiation factor;
- Leukemia inhibitory factor;
Abstract: The neurotransmitter phenotype switch that occurs in cultures of rat superior cervical ganglion neurons after treatment with leukemia inhibitory factor or ciliary neurotrophic factor is a useful model permitting investigation of the mechanisms of cytokine-mediated differentiation. Recently the actions of leukemia inhibitory factor and ciliary neurotrophic factor have been linked through their interactions with related receptor complexes. Here we compare the effects of these two cytokines on gene expression in sympathetic neuronal cultures and begin to investigate their mechanisms. We report that, as has been shown for leukemia inhibitory factor, ciliary neurotrophic factor regulates peptides and classical transmitters in these cultures at the mRNA level. In addition, we find that the induction of substance P mRNA by these cytokines is rapid, dependent on protein synthesis, and occurs in 40–50% of superior cervical ganglion neurons in dissociated culture.