The current address of Dr. H. Martin is Proteus Molecular Design Ltd., Proteus House, Lyme Green Business Park, Macclesfield, Cheshire SK11 OJL, U.K.
Subcellular Localisation of 14-3-3 Isoforms in Rat Brain Using Specific Antibodies
Version of Record online: 23 NOV 2002
Journal of Neurochemistry
Volume 63, Issue 6, pages 2259–2265, December 1994
How to Cite
Martin, H., Rostas, J., Patel, Y. and Aitken, A. (1994), Subcellular Localisation of 14-3-3 Isoforms in Rat Brain Using Specific Antibodies. Journal of Neurochemistry, 63: 2259–2265. doi: 10.1046/j.1471-4159.1994.63062259.x
- Issue online: 23 NOV 2002
- Version of Record online: 23 NOV 2002
- Received December 23, 1993; revised manuscript received March 23, 1994; accepted April 11, 1994.
- 14-3-3 proteins;
- Isoform-specific antibodies
Abstract: The 14-3-3 protein family, which is present at particularly high concentrations in mammalian brain, is known to be involved in various cellular functions, including protein kinase C regulation and exocytosis. Despite the fact that most of the 14-3-3 proteins are cytosolic, a small but significant proportion of 14-3-3 in brain is tightly and selectively associated with some membranes. Using a panel of isoform-specific antisera we find that the ε, η, γ, β, and ζ isoforms are all present in purified synaptic membranes but absent from mitochondrial and myelin membranes. In addition, the η, ε, and γ isoforms but not the β and ζ isoforms are associated with isolated synaptic junctions. When different populations of synaptosomes were fractionated by a nonequilibrium Percoll gradient procedure, the ε and γ isoforms were present and the β and ζ isoforms were absent from the membranes of synaptosomes sedimenting in the more dense parts of the gradient. The finding that these proteins are associated with different populations of synaptic membranes suggests that they are selectively expressed in different classes of neurones and raises the possibility that some or all of them may influence neurotransmission by regulating exocytosis and/or phosphorylation.