The nucleotide sequences reported in this study have been deposited in the EMBL database with accession nos. X94343 (chicken) and X94342 (goldfish).
Conservation of γ-Aminobutyric Acid Type A Receptor α6 Subunit Gene Expression in Cerebellar Granule Cells†
Article first published online: 23 NOV 2002
Journal of Neurochemistry
Volume 66, Issue 5, pages 1810–1818, May 1996
How to Cite
Bahn, S., Harvey, R. J., Darlison, M. G. and Wisden, W. (1996), Conservation of γ-Aminobutyric Acid Type A Receptor α6 Subunit Gene Expression in Cerebellar Granule Cells. Journal of Neurochemistry, 66: 1810–1818. doi: 10.1046/j.1471-4159.1996.66051810.x
- Issue published online: 23 NOV 2002
- Article first published online: 23 NOV 2002
- Received November 11, 1995; revised manuscript received December 21, 1995; accepted December 21, 1995.
- Teleost fish;
- Granule cell;
Abstract: The γ-aminobutyric acid type A receptor cDNAs encoding the α6 subunit homologues from chicken and goldfish have been cloned and sequenced. These proteins exhibit 83 and 75% identity, respectively, to the rat α6 polypeptide. In situ hybridization has demonstrated that, as in mammals, the avian and teleost fish α6 subunit genes are predominately expressed in cerebellar granule cells. Correspondingly, flunitrazepam-nondisplaceable binding of [3H]Ro 15-4513 (a benzodiazepine partial inverse agonist), which is a major characteristic of γ-aminobutyric acid type A receptors that contain the α6 polypeptide, is also mainly found for cerebellar granule cells of fish and chick. The conservation of this expression pattern suggests that γ-aminobutyric acid type A receptors possessing the α6 subunit are of fundamental importance for cerebellar function and that the corresponding gene regulatory elements, e.g., granule cell-specific enhancers, have also been conserved.