The present address of Dr. B. K. Wagner and Dr. J. A. Broderick is Department of Biomedical Sciences, E. K. Shriver Center for Mental Retardation, Waltham, MA 02254, U.S.A.
A τ Promoter Region Without Neuronal Specificity
Article first published online: 23 NOV 2002
Journal of Neurochemistry
Volume 66, Issue 6, pages 2257–2263, June 1996
How to Cite
Andreadis, A., Wagner, B. K., Broderick, J. A. and Kosik, K. S. (1996), A τ Promoter Region Without Neuronal Specificity. Journal of Neurochemistry, 66: 2257–2263. doi: 10.1046/j.1471-4159.1996.66062257.x
- Issue published online: 23 NOV 2002
- Article first published online: 23 NOV 2002
- Received September 14, 1995; revised manuscript received December 14, 1995; accepted January 23, 1996.
- Microtubule-associated protein τ;
- 5′-Untranslated region;
- Homogeneous initiation sites;
- Promoter mapping;
- Specificity of expression
Abstract: The microtubule-associated protein τ is produced from a 6-kb mRNA expressed primarily in neurons. A 2-kb τ mRNA has also been characterized, which produces a τ isoform that localizes to the nucleus, and an 8-kb mRNA is expressed in the PNS. Mapping and sequencing of the human τ gene start showed that it has an unusually GC-rich 5′-untranslated region coded by a single untranslated exon (designated −1). Primer extensions and expression assays indicated that upstream of exon −1 is a promoter that is not neuron specific. This region contains consensus binding sites for transcription factors AP2, Sp1, and GCF.