Cellular Distribution of 6-Phosphofructo-1-Kinase Isoenzymes in Rat Brain
Version of Record online: 23 NOV 2002
Journal of Neurochemistry
Volume 67, Issue 6, pages 2573–2580, December 1996
How to Cite
Zeitschel, U., Bigl, M., Eschrich, K. and Bigl, V. (1996), Cellular Distribution of 6-Phosphofructo-1-Kinase Isoenzymes in Rat Brain. Journal of Neurochemistry, 67: 2573–2580. doi: 10.1046/j.1471-4159.1996.67062573.x
- Issue online: 23 NOV 2002
- Version of Record online: 23 NOV 2002
- Received May 17, 1996; revised manuscript received July 30, 1996; accepted July 30, 1996.
- 6-Phosphofructo-1-kinase isoenzymes;
- Rat brain;
- Cell culture;
- Cell isolation;
- Western blotting;
Abstract: In the brain, all three isoenzyme types [muscle (M), liver (L), and brain (C)] of 6-phosphofructo-1-kinase (PFK; EC 220.127.116.11) occur, forming a complex mixture of homo- and heterotetramers. The PFK isoenzyme pattern of the different brain cell types is yet unknown. In the present study, we investigated the distribution of the PFK isoenzyme subunits in primary and secondary cell cultures and in bulk-isolated cells of rat brain by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting. All three PFK isoenzymes are present in all cell types but in different proportions. The cellular distribution of the PFK isoenzymes in situ was studied immunohistochemically with different polyclonal antisera against purified rat PFKs. The monospecific antibody against M-type PFK stained preferentially the perinuclear areas of neurons and glial cells. The antibodies that in immunoblots detected mainly the L-type PFK showed a characteristic staining in only the cytoplasma and the processes of cells, whereas the C-type antibodies almost homogeneously stained whole cell bodies as well as large dendrites. Because the PFK isoenzymes differ with respect to their allosteric properties, their differential distribution in different brain cells might be of importance for the regulation of brain glycolysis in the different cellular compartments of the brain.