Cloning and Characterization of a Mouse σ1 Receptor
Article first published online: 13 NOV 2002
Journal of Neurochemistry
Volume 70, Issue 6, pages 2279–2285, June 1998
How to Cite
Pan, Y.-X., Mei, J., Xu, J., Wan, B.-L., Zuckerman, A. and Pasternak, G. W. (1998), Cloning and Characterization of a Mouse σ1 Receptor. Journal of Neurochemistry, 70: 2279–2285. doi: 10.1046/j.1471-4159.1998.70062279.x
- Issue published online: 13 NOV 2002
- Article first published online: 13 NOV 2002
- Received December 9, 1997; revised manuscript received January 8, 1998; accepted January 8, 1998.
- σ receptor;
Abstract: A cDNA clone (S2-1a) isolated from a mouse brain cDNA library, using a guinea pig σ1 cDNA as probe, has high homology to the predicted protein sequence of the guinea pig (88%) and human (90%) σ1 receptors. Northern analysis revealed a major mRNA of ∼1.8 kb in a wide range of mouse tissues, with highest levels in brain, liver, kidney, and thymus. Southern analysis and chromosomal mapping in the mouse suggested a single-copy gene in region A5-B2 of chromosome 4. Expression of the clone in MCF-7 and CHO cells led to a pronounced increase in (+)-[3H]pentazocine binding with a selectivity profile consistent with σ1 receptors. In vitro translation yielded a protein of ∼28 kDa, as did transfection of a probe containing the hemagglutinin (HA) epitope (S2-1a.HA) into CHO cells, as determined by western analysis using an antibody directed against HA. (+)-[3H]-Pentazocine binding to immunopurified HA-tagged receptor demonstrated conclusively that S2-1a.HA encodes a high-affinity (+)-[3H]pentazocine binding site with characteristics of a murine σ1 receptor. An antisense oligodeoxynucleotide designed from S2-1a potentiated opioid analgesia in vivo.