Enhancement of Extracellular Glutamate Scavenge System in Injured Motoneurons


Address correspondence and reprint requests to Dr. H. Kiyama at Department of Anatomy, Asahikawa Medical College, 4-5-3-11 Nishikagura, Asahikawa, Hokkaido, 078-8510, Japan.


Abstract: An increase in glutamine synthetase (GS) mRNA expression after peripheral motor nerve injury was demonstrated by differential display PCR using single arbitrary primer coupled with in situ hybridization screening called in situ display. Differential display PCR was carried out to compare differences in mRNA expression between axotomized (6 h after the transection) and normal hypoglossal nuclei in mice. Several gene fragments were increased after nerve injury; one was identified as GS. Subsequent emulsion autoradiography of hybridization tissue sections revealed that the increase in GS mRNA was observed in injured motoneurons. As GS is a key enzyme participating in the metabolism of the major excitatory neurotransmitter glutamate, we examined the significance of increased GS expression on glutamate-uptake kinetics. GS-transfected human embryonic kidney cells showed an up-regulation in glutamate-uptake kinetics. Therefore, newly expressed GS together with an increased expression of the neuronal glutamate transporter EAAC1 in the injured motoneurons accelerates glutamate uptake. The present results may suggest that the glutamate-uptake system involving the neuronal glutamate transporter and GS in injured neurons is enhanced so as to provide resistance against neurotoxic glutamate accumulation during the early process of nerve regeneration.

Abbreviations used: DD-PCR, differential display PCR; GS, glutamine synthetase; HEK, human embryonic kidney; PB, phosphate buffer; SDS, sodium dodecyl sulfate; SSC, saline-sodium citrate buffer; SSPE, standard saline-phosphate-EDTA buffer; TB, transport buffer.