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Acknowledgements

We thank Connie Russell and Dr. M. Burg for their earlier work with a cosmid clone and Dr. G. Koliantz for his help in cytological experiments. We express our gratitude to Drs. T. C. Kaufman, P. Salvaterra, J. Tamkun, G. Cutting, and R. ffrench-Constant for kindly providing us with the DNA clones used in our study. Ann Pellegrino assisted in the preparation of the manuscript. This work was supported by grant MH 49727 from the National Institute of Mental Health and grant EY00033 from the National Eye Institute.

  • 1
    Adelman J.P., Shen K.Z., Kavanaugh M.P., Warren R.A., Wu Y.N., Lagrutta A., Bond C.T., North R.A. (1992) Calcium-activated potassium channels expressed from cloned complementary DNAs.Neuron 9,209216.
  • 2
    Bettler B., Boulter J., Hermans-Borgmeyer I., O'Shea-Greenfield A., Deneris E.S., Moll C., Borgmeyer U., Hollman M., Heinemann S. (1990) Cloning of a novel glutamate receptor subunit, GluR5 : expression in the nervous system during development.Neuron 5,583595.
  • 3
    Burnashev N., Monyer H., Seeburg P.H., Sakmann B. (1992) Divalent ion permeability of AMPA receptor channels is dominated by the edited form of a single subunit.Neuron 8,189198.
  • 4
    Cully D.F., Vassiliatis D.K., Liu K.K., Paress P.S., Van der Ploeg P.S., Schaeffer J.M., Arena J.P. (1994) Cloning of an avermectin-sensitive glutamate-gated chloride channel fromCaenorhabditis elegans. Nature 371,707711.
  • 5
    Cully D.F., Paress P.S., Liu K.K., Schaeffer J.M., Arena J.P. (1996) Identification of a Drosophila melanogaster glutamate-gated chloride channel sensitive to the antiparasitic agent avermectin.J. Biol. Chem. 271,2018720191.
  • 6
    Cutting G.R., Lu L., O'Hara B.F., Kasch L.M., Montrose-Rafizadeh C., Donoman D.M., Shimada S., Antonarakis S.E., Guggino W.B., Uhl G.R., Kazazian H.H.J r. (1991) Cloning of the γ-aminobutyric acid (GABA) r1 cDNA : a GABA receptor subunit highly expressed in the retina.Proc. Natl. Acad. Sci. USA 88,26732677.
  • 7
    Feinberg A.P. & Vogelstein B. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.Anal. Biochem. 132,613.
  • 8
    Ffrench-Constant R.H. & Rocheleau T.A. (1993) Drosophilaγ-aminobutyric acid receptor gene Rdl shows extensive alternative splicing.J. Neurochem. 60,23232326.
  • 9
    Ffrench-Constant R.H., Mortlock D.P., Shaffer C.D., MacIntyre R.J., Roush R.T. (1991) Molecular cloning and transformation of cyclodiene resistance in Drosophila : an invertebrate gamma-aminobutyric acid subtype A receptor locus.Proc. Natl. Acad. Sci. USA 88,72097213.
  • 10
    Gall J.G. & Pardue M.L. (1971) Nucleic acid hybridization in cytological preparations.Methods Enzymol.21,470480.
  • 11
    Gallo V., Upson L.M., Hayes W.P., Vyklicky L., Winters C.A., Buonanno A. (1992) Molecular cloning and developmental analysis of a new glutamate receptor subunit isoform in cerebellum.J. Neurosci. 12,10101023.
  • 12
    Hirose T., Fan H., Suzuki J.Y., Wakasugi T., Tsudzuki T., Kossel H., Sigura M. (1996) Occurrence of silent RNA editing in chloroplasts : its species-specificity and the influence of environmental and developmental conditions.Plant Mol. Biol. 30,667672.
  • 13
    Hofmann F., Biel M., Flockerzi V. (1994) Molecular basis for Ca2+ channel diversity.Annu. Rev. Neurosci. 17,399418.
  • 14
    Hollman M. & Heinemann S. (1994) Cloned glutamate receptors.Annu. Rev. Neurosci. 17,31108.
  • 15
    Kao P.N. & Karlin A. (1986) Acetylcholine receptor binding site contains a disulfide crosslink between adjacent half-cystinil residues.J. Biol. Chem. 261,80858088.
  • 16
    Karlin A. & Akabas M.H. (1995) Toward a structural basis for the function of nicotinic acetylcholine receptors and their cousins.Neuron 15,12311244.
  • 17
    Kempken F., Hofken G., Pring D.R. (1995) Analysis of silent RNA editing sites in atp6 transcripts of Sorghum bicolor.Curr. Genet. 27,555558.
  • 18
    Kohler M., Burnashev N., Sakmann B., Seeburg P.H. (1993) Determinants of Ca2+ permeability in both TM1 and TM2 of high affinity kainate receptor channels : diversity by RNA editing.Neuron 10,491500.
  • 19
    Laughton D.L., Lunt G.G., Wolstenholme A.J. (1997) Alternative splicing of a Caenorhabditis elegans gene produces two novel inhibitory amino acid receptor subunits with identical ligand binding domains but different ion channels.Gene 201,119125.DOI: 10.1016/S0378-1119(97)00436-8
  • 20
    Lee H.J., Rochelau T., Zhang H.G., Jackson M.B., -Constant R.H. (1993) Expression of a Drosophila GABA receptor in a baculovirus insect cell system. Functional expression of insecticide susceptible and resistant GABA receptors from the cyclodiene resistance geneRdl. FEBS Lett. 335,315318.
  • 21
    Loughney K., Kreber R., Ganetzky B. (1989) Molecular analysis of the para locus, a sodium channel gene in Drosophila.Cell 58,11431154.
  • 22
    Maas S., Melcher T., Herb A., Seeburg P.H., Keller W., Krause S., Higuchi M., O'Connel M.A. (1996) Structural requirements for RNA editing in glutamate receptor pre-mRNAs by recombinant double-stranded RNA adenosine deaminase.J. Biol. Chem. 271,1222112226.
  • 23
    Nakanishi S. (1992) Molecular diversity of glutamate receptors and implications for brain function.Science 258,597603.
  • 24
    Petschek J.P., Mermer M.J., Scheckelhoff M.R., Simone A.A., Vaughn J.C. (1996) RNA editing in Drosophila 4f-rnp gene nuclear transcripts by multiple A-to-G conversions.J. Mol. Biol. 259,885890.DOI: 10.1006/jmbi.1996.0365
  • 25
    Pongs O., Kecskemethy N., Muller R., Krah-Jentgens I., Baumann A., Kiltz H.H., Canal I., Llamazares S., Ferrus A. (1988) Shaker encodes a family of putative potassium channel proteins in the nervous system of Drosophila.EMBO J. 7,10871096.
  • 26
    Rabow L.E., Russek S.J., Farb D.H. (1995) From ion currents to genomic analysis : recent advances in GABAA receptor research.Synapse 21,189274.
  • 27
    Sambrook J., Fritsch E.F., Maniatis T. (1989) Molecular Cloning : A Laboratory Manual. Cold Spring Harbor Press, Cold Spring Harbor, New York.
  • 28
    Schuster C., Ultsch A., Schloss P., Cox J.A., Schmitt B., Betz H. (1991) Molecular cloning of an invertebrate glutamate receptor subunit expressed in Drosophila muscle.Science 254,112114.
  • 29
    Schwartz T.L., Tempel B.L., Papazian D.M., Jan Y.N., Jan L.Y. (1988) Multiple potassium-channel components are produced by alternative splicing at the Shaker locus in Drosophila.Nature 331,137142.
  • 30
    Seeburg P.H. (1996) The role of RNA editing in controlling glutamate receptor channel properties.J. Neurochem. 66,15.
  • 31
    Semenov E.P. & Pak W.L. (1996) A novel Drosophila chloride channel gene showing high homology with human Rho genes.Soc. Neurosci. Abstr. 22,138.17.
  • 32
    Smith L.A., Wang X., Peixoto A.A., Neumann E.K., Hall L.M., Hall J.C. (1996) A Drosophila calcium channel a1 subunit gene maps to a genetic locus associated with behavioral and visual difects.J. Neurosci. 16,78687879.
  • 33
    Sommer B. & Seeburg P.H. (1992) Glutamate receptor channels—novel properties and new clones. Trends Pharmacol. Sci. 13,291296.
  • 34
    Sommer B., Keinanen K., Verdoorn T.A., Wisden W., Burnashev N., Herb A., Kohler M., Takagi T., Sakmann B., Seeburg P.H. (1990) Flip and flop : a cell-specific functional switch in glutamate-operated channels of the CNS.Science 249,15801585.
  • 35
    Sommer B., Kohler M., Sprengel R., Seeburg P.H. (1991) RNA editing in brain controls a determinant of ion flow in glutamategated channels.Cell 67,1119.
  • 36
    Sommer B., Burnashev N., Verdoorn T.A., Sakmann B., Seeburg P.H. (1992) A glutamate receptor channel with high affinity for domoate and kainate.EMBO J.4,16511656.
  • 37
    Stea A., Soong T.W., Snutch T.P. (1995) Voltage-gated calcium channels, inLigand- and Voltage-Gated Ion Channels (North R. A., ed), pp. 114151. CRC Press, Boca Raton, Florida.
  • 38
    Ultsch A., Schuster C.M., Laube B., Schloss P., Schmitt B., Betz H. (1992) Glutamate receptors of Drosophila melanogaster : cloning of a kainate-selective subunit expressed in the central nervous system.Proc. Natl. Acad. Sci. USA 89,1048410488.