We thank Dr. I. A. Romero for the critical reading of the manuscript and I. Bouchaert for technical support with the confocal microscopy. This study was supported by the Programa Praxis XXI of the Fundaçao para a Ciência e Tecnologia and Programa Gulbenkian de Doutoramento em Biologia e Medicina, the Centre National de la Recherche Scientifique, the Institut National de la Recherche Médicale, the Association pour le Développement de la Recherche sur le Cancer, the Ligue Nationale Française contre le Cancer, the Université de Paris, and the Ministère de la Recherche et de L'Enseignement Supérieur.

  • 1
    Anderson R.G.W. (1993) Caveolae : where incoming and outgoing messengers meet.Proc. Natl. Acad. Sci. USA 90,1090910913.
  • 2
    Baynash A.G., Hosoda K., Giaid A., Richardson J.A., Emoto N., Hammer R.E., Yanagisawa M. (1994) Interaction of endothelin-3 with endothelin B receptor is essential for development of epidermal melanocytes and enteric neurons.Cell 79,12771285.
  • 3
    Bouillot C., Prochiantz A., Rougon G., Allinquant B. (1996) Axonal amyloid percursor protein expressed by neurons in vitro is present in a membrane fraction with caveolae-like properties.J. Biol. Chem. 271,76407644.
  • 4
    Bradley M.P., Rayns D.G., Forrester I.T. (1980) Effects of filipin, digitonin and polymyxin b on plasma membrane of ram spermatozoa—an EM study. Arch. Androl. 4,195204.
  • 5
    Cameron P.L., Ruffin J.W., Bollag R., Rasmussen H., Cameron R.S. (1997) Identification of caveolin and caveolin-related proteins in the brain.J. Neurosci. 17,95209535.
  • 6
    Cazaubon S., Parker P.J., Strosberg A.D., Couraud P.O. (1993) Endothelins stimulate tyrosine phosphorylation and activity of p42/mitogen-activated protein kinase in astrocytes.Biochem. J. 293,381386.
  • 7
    Cazaubon S.M., Ramos-Morales F., Fischer S., Schweighoffer F., Strosberg A.D., Couraud P.O. (1994) Endothelin induces tyrosine phosphorylation and GRB2 association of Shc in astrocytes.J. Biol. Chem. 269,2480524809.
  • 8
    Cazaubon S., Chaverot N., Romero I.A., Girault J.A., Adamson P., Strosberg A.D., Couraud P.O. (1997) Growth factor activity of endothelin-1 in primary astrocytes mediated by adhesion-dependent and -independent pathways.J. Neurosci. 17,62036212.
  • 9
    Chun M., Liyanage U.K., Lisanti M.P., Lodish H.F. (1994) Signal transduction of a G protein-coupled receptor in caveolae : colocalization of endothelin and its receptor with caveolin.Proc. Natl. Acad. Sci. USA 91,1172811732.
  • 10
    Couraud P.O., Durieu-Trautmann O., Le Nguyen D., Marin P., Glibert F., Strosberg A.D. (1991) Functional endothelin-1 receptors in rat astrocytoma C6.Eur. J. Pharmacol. 206,191198.
  • 11
    Craig S.W. & Johnson R.P. (1996) Assembly of focal adhesions : progress, paradigms, and portents.Curr. Opin. Cell Biol. 8,7485.
  • 12
    Durieu-Trautmann O., Fédérici C., Créminon C., Foignant-Chaverot N., Roux F., Claire M., Strosberg A.D., Couraud P.O. (1993) Nitric oxide and endothelin secretion by microvessel endothelial cells : regulation by cyclic nucleotides.J. Cell. Physiol. 155,104111.
  • 13
    Ehrenreich H., Rieckmann P., Sinowatz F., Weih K.A., Arthur L.O., Goebel F., Burd P.R., Coligan J.E., Clouse K.A. (1993) Potent stimulation of monocytic endothelin-1 production by HIV-1 glycoprotein 120.J. Immunol. 150,46014609.
  • 14
    Engelman J.A., Wykoff C.C., Yasuhara S., Song K.S., Okamoto T., Lisanti M.P. (1997) Recombinant expression of caveolin-1 in oncogenically transformed cells abrogates anchorage-independent growth.J. Biol. Chem. 272,1637416381.
  • 15
    Hotchin N.A. & Hall A. (1995) The assembly of integrin adhesion complexes requires both extracellular matrix and intracellular rho/rac GTPases.J. Cell. Biol. 131,18571865.
  • 16
    Koleske A.J., Baltimore D., Lisanti M.P. (1995) Reduction of caveolin and caveolae in oncogenically transformed cells.Proc. Natl. Acad. Sci. USA 92,13811385.
  • 17
    Kurihara Y., Kurihara H., Suzuki H., Kodama T., Maemura K., Nagai R., Oda H., Kuawki T., Cao W.H., Kamada N., Jishage K., Ouchi Y., Azuma S., Toyoda Y., Ishikawa T., Kumada M., Yazaki Y. (1994) Elevated blood pressure on craniofacial abnormalities in mice deficient in endothelin-1.Nature 368,703710.
  • 18
    Lazarini F., Strosberg A.D., Couraud P.O., Cazaubon S.M. (1996) Coupling of ETB endothelin receptor to mitogen-activated protein kinase stimulation and DNA synthesis in primary cultures of rat astrocytes.J. Neurochem. 66,459465.
  • 19
    Lisanti M.P., Scherer P.E., Tang Z., Sargiacomo M. (1994) Caveolae, caveolin and caveolin-rich membrane domains : a signaling hypothesis.Trends Cell Biol. 4,231235.
  • 20
    Liu J., Oh P., Horner T., Rogers R.A., Schnitzer J.E. (1997) Organized endothelial cell surface signal transduction in caveolae distinct from glycosylphosphatidylinositol-anchored protein microdomains.J. Biol. Chem. 272,72117222.
  • 21
    Liu P., Ying Y., Ko Y., Anderson R.G.W. (1996) Localization of platelet-derived growth factor-stimulated phosphorylation cascade to caveolae.J. Biol. Chem. 271,1029910303.
  • 22
    Liu P., Ying Y., Anderson R.G.W. (1997) Platelet-derived growth factor activates mitogen-activated protein kinase in isolated caveolae.Proc. Natl. Acad. Sci. USA 94,1366613670.
  • 23
    Mineo C., James G.L., Smart E.J., Anderson R.G.W. (1996) Localization of epidermal growth factor-stimulated Ras/Raf-1 interaction to caveolae membrane.J. Biol. Chem. 271,1193011935.
  • 24
    Okamoto T., Schlegel A., Scherer P.E., Lisanti M.P. (1998) Caveolins, a family of scaffolding proteins for organizing “preassembled signaling complexes” at the plasma membrane.J. Biol. Chem. 273,54195422.
  • 25
    Olive S., Dubois C., Schachner M., Rougon G. (1995) The F3 neuronal glycosylphosphatidylinositol-linked molecule is localized to glycolipid-enriched membrane subdomains and interacts with L1 and Fyn kinase in cerebellum.J. Neurochem. 65,23072317.
  • 26
    Orlandi P.A. & Fishman P.H. (1998) Filipin-dependent inhibition of cholera toxin : evidence for toxin internalization and activation through caveolae-like domains.J. Cell Biol. 141,905915.
  • 27
    Parton R.G. (1996) Caveolae and caveolins.Curr. Opin. Cell Biol. 8,542548.
  • 28
    Rothberg K.R., Heuser J.E., Donzell W.C., Ying Y., Glenney J.R., Anderson R.G.W. (1992) Caveolin, a protein component of caveolae membrane coats.Cell 68,673682.
  • 29
    Sargiacomo M., Scherer P.E., Tang Z.L., Casanova J.E., Lisanti M.P. (1994) In vitro phosphorylation of caveolin-rich membrane domains : identification of an associated serine kinase activity as a casein kinase II-like enzyme.Oncogene 9,25892595.
  • 30
    Scherer P.E., Okamoto T., Chun M., Nishimoto I., Lodish H.F., Lisanti M.P. (1996) Identification, sequence, and expression of caveolin-2 defines a caveolin gene family.Proc. Natl. Acad. Sci. USA 93,131135.
  • 31
    Schnitzer J.E., Oh P., Pinney E., Allard J. (1994) Filipin-sensitive caveolae-mediated transport in endothelium : reduced transcytosis, scavenger endocytosis, and capillary permeability of selected macromolecules.J. Cell Biol 127,12171232.
  • 32
    Smart E.J., Mineo C., Anderson R.G. (1996) Clustered folate receptors deliver 5-methyltetrahydrofolate to cytoplasm of MA104 cells.J. Cell. Biol 134,11691177.
  • 33
    Song K.S., Shengwen L., Okamoto T., Quilliam L.A., Sargiacomo M., Lisanti M.P. (1996) Co-purification and direct interaction of Ras with Caveolin an integral membrane protein of caveolae microdomains.J. Biol. Chem 271,96909697.
  • 34
    Wu C., Butz S., Ying Y., Anderson G.W. (1997) Tyrosine kinase receptors concentrated in caveolae-like domains from neuronal plasma membrane.J. Biol. Chem 272,35543559.
  • 35
    Yamashita K., Niwa M., Kataoka Y., Shigematsu K., Himeno A., Tsutsumi K., Nakano-Nakashima M., Sakurai-Yamashita Y., Shibata S., Taniyama K. (1994) Microglia with an endothelin ETB receptor aggregate in rat hippocampus CA1 subfields following transient forebrain ischemia.J. Neurochem 63,10421051.