The GenBank accession numbers for the glutamate receptor subunits/subtype are as follows : GluR1-4, M38060-38063 ; NMDAR1, X63255 ; mGluR5, D10891.
Neuroadaptations in Ionotropic and Metabotropic Glutamate Receptor mRNA Produced by Cocaine Treatment
Article first published online: 18 JAN 2002
Journal of Neurochemistry
Volume 72, Issue 1, pages 157–165, January 1999
How to Cite
Ghasemzadeh, M. B., Nelson, L. C., Lu, X.-Y. and Kalivas, P. W. (1999), Neuroadaptations in Ionotropic and Metabotropic Glutamate Receptor mRNA Produced by Cocaine Treatment. Journal of Neurochemistry, 72: 157–165. doi: 10.1046/j.1471-4159.1999.0720157.x
Abbreviations used : AMPA, (±)-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ; GluR, glutamate receptor ; mGluR, metabotropic glutamate receptor ; NAcore, nucleus accumbens core ; NAshell, nucleus accumbens shell ; NMDA, N-methyl-d-aspartate ; NMDAR, N-methyl-d-aspartate receptor ; TAE, Tris-acetate-EDTA ; VTA, ventral tegmental area.
- Issue published online: 18 JAN 2002
- Article first published online: 18 JAN 2002
- Glutamate receptor;
- Nucleus accumbens;
- Prefrontal cortex;
- Ventral tegmental area;
Abstract : The expression of glutamate receptor/subunit mRNAs was examined 3 weeks after discontinuing 1 week of daily injections of saline or cocaine. The level of mRNA for GluR1-4, NMDAR1, and mGluR5 receptors was measured with in situ hybridization and RT-PCR. In nucleus accumbens, acute cocaine treatment significantly reduced the mRNA level for GluR3, GluR4, and NMDAR1 subunits, whereas repeated cocaine reduced the level for GluR3 mRNA. Acute cocaine treatment also reduced the NMDAR1 mRNA level in dorsolateral striatum and ventral tegmental area. In prefrontal cortex, repeated cocaine treatment significantly increased the level of GluR2 mRNA. The GluR2 mRNA level was not changed by acute or repeated cocaine in any other brain regions examined. Repeated cocaine treatment also significantly increased mGluR5 mRNA levels in nucleus accumbens shell and dorsolateral striatum. Functional properties of the ionotropic glutamate receptors are determined by subunit composition. In addition, metabotropic glutamate receptors can modulate synaptic transmission and the response to stimulation of ionotropic receptors. Thus, the observed changes in levels of AMPA and NMDA receptor subunits and the mGluR5 metabotropic receptor may alter excitatory neurotransmission in the mesocorticolimbic dopamine system, which could play a significant role in the enduring biochemical and behavioral effects of cocaine.