Isolation of 10 Differentially Expressed cDNAs in Differentiated Neuro2a Cells Induced Through Controlled Expression of the GD3 Synthase Gene
Article first published online: 28 JUN 2008
Journal of Neurochemistry
Volume 72, Issue 5, pages 1781–1790, May 1999
How to Cite
Liu, H., Nakagawa, T., Kanematsu, T., Uchida, T. and Tsuji, S. (1999), Isolation of 10 Differentially Expressed cDNAs in Differentiated Neuro2a Cells Induced Through Controlled Expression of the GD3 Synthase Gene. Journal of Neurochemistry, 72: 1781–1790. doi: 10.1046/j.1471-4159.1999.0721781.x
- Issue published online: 28 JUN 2008
- Article first published online: 28 JUN 2008
- Received October 13, 1998; revised manuscript received December 31, 1998; accepted December 31, 1998.
- GD3 synthase gene;
- Neuro2a cells;
- P19 cells;
- Neural differentiation;
- mRNA differential display
Abstract: Recently, we showed that transfection of GD3 synthase cDNA into Neuro2a cells, a mouse neuroblastoma cell line, causes cell differentiation with neurite sprouting. In a search for the genes involved in this ganglioside-induced Neuro2a differentiation, we used a tetracycline-regulated GD3 synthase cDNA expression system combined with differential display PCRs to identify mRNAs that were differentially expressed at four representative time points during the process. We report here the identification of 10 mRNAs that are expressed highly at the Neuro2a differentiated stage. These cDNAs were named GDAP1–GDAP10 for (ganglioside-induced differentiation-associated protein) cDNAs. It is interesting that in retinoic acid-induced neural differentiated mouse embryonic carcinoma P19 cells, GDAP mRNA expression levels were also up-regulated (except that of GDAP3), ranging from three to > 10 times compared with nondifferentiated P19 cells. All the GDAP genes (except that of GDAP3) were developmentally regulated. The GDAP1, 2, 6, 8, and 10 mRNAs were expressed highly in the adult mouse brain, whereas all the other GDAP mRNAs were expressed in most tissues. Our results suggested that these GDAP genes might be involved in the signal transduction pathway that is triggered through the expression of a single sialyltransferase gene to induce neurite-like differentiation of Neuro2a cells.