Lippincott Williams & Wilkins, Inc., Philadelphia
Radicicol Potentiates Neurotrophin-Mediated Neurite Outgrowth and Survival of Cultured Sensory Neurons from Chick Embryo
Article first published online: 18 JAN 2002
Journal of Neurochemistry
Volume 72, Issue 6, pages 2256–2262, June 1999
How to Cite
Sano, M., Yoshida, M., Fukui, S. and Kitajima, S. (1999), Radicicol Potentiates Neurotrophin-Mediated Neurite Outgrowth and Survival of Cultured Sensory Neurons from Chick Embryo. Journal of Neurochemistry, 72: 2256–2262. doi: 10.1046/j.1471-4159.1999.0722256.x
Abbreviations used: BDNF, brain-derived neurotrophic factor; DRG, dorsal root ganglion; HSP90, 90-kDa heat shock protein; MAP, mitogen-activated protein; NGF, nerve growth factor; NT-3, neurotrophin-3; PAGE, polyacrylamide gel electrophoresis; P13-kinase, phosphatidylinositol 3-kinase; SDS, sodium dodecyl sulfate; TUNEL, terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end-labeling.
- Issue published online: 18 JAN 2002
- Article first published online: 18 JAN 2002
- Nerve growth factor;
- Brain-derived neurotrophic factor;
- Sensory neuron;
- Survival factor
Abstract: Radicicol, an antifungal antibiotic with markedly low toxicity, is a potent inhibitor of the Src family of protein tyrosine kinases and causes morphological reversion of v-src-transformed fibroblasts. Recently, this antibiotic was also found to inhibit Raf kinase. In the present study, we found that nanomolar concentrations of radicicol (10 ng/ml) enhanced the survival and neurite outgrowth of neurons from embryonic chick dorsal root ganglia (DRGs) and sympathetic ganglia. It potentiated the trophic effects of nerve growth factor, brain-derived neurotrophic factor, and neurotrophin-3 on the cultured DRG neurons. This concentration of radicicol did not alter the tyrosine phosphorylation of Trk receptors or the activity of mitogen-activated protein (MAP) kinases. Wortmannin, an inhibitor of phosphatidylinositol 3-kinase (P13-kinase), did not inhibit radicicol, excluding the involvement of P13-kinase in the radicicol-dependent trophic actions. These results suggest that radicicol mediates neuronal growth presumably via a mechanism not involving the activation of Trk receptors, MAP kinase, or P13-kinase.