This research was supported by grant 10680735 from the Ministry of Education, Science and Culture of Japan.

  • 1
    Batistatou A. & Greene I.A. (1991) Aurintricarboxylic acid rescues PC12 cells and sympathetic neurons from cell death caused by nerve growth factor deprivation: correlation with suppression of endonuclease activity.J. Cell Biol. 15 461471.
  • 2
    Borasio G.D. (1990) Differential effects of protein kinase inhibitor K-252a on the in vitro survival of chick embryonic neurons.Neurosci. Lett. 108 207212.
  • 3
    Borasio G.D., John J., Wittinghofer A., Barde Y., Sendtner M., Heumann R. (1989) Ras p21 protein promotes survival and fiber outgrowth of cultured embryonic neurons.Neuron 2 10871096.
  • 4
    Bradford M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal. Biochem. 72 248254.
  • 5
    Buchman V.L. & Davies A.M. (1993) Different neurotrophins are expressed and act in a developmental sequence to promote the survival of embryonic sensory neurons.Development 118 9891001.
  • 6
    Chanmugam P., Feng L., Liou S., Jang B.C., Boudreau M., Yu G., Lee J.H., Kwon H.J., Beppu T., Yoshida M., Xia Y., Wilson C.B., Hwang D. (1995) Radicicol, a potent protein tyrosine kinase inhibitor, suppresses the expression of mitogen-induced cyclooxygenase in macrophages stimulated with lipopolysaccharide and in experimental glomerulonephritis.J. Biol. Chem. 270 54185426.
  • 7
    Coco Maroney A., Lipfert L., Forbes M.E., Glicksman M.A., Neff N.T., Siman R., Dionne C.A. (1995) K-252a induces tyrosine phosphorylation of the focal adhesion kinase and neurite outgrowth in human neuroblastoma SH-SY5Y cells.J. Neurochem. 64 540549.
  • 8
    Cowley S., Paterson H., Kemp P., Marshall J. (1994) Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and transformation of NIH 3T3 cells.Cell 77 841852.
  • 9
    Delmotte P. & Delmotte-Plaquee J.A. (1953) A new antifungal substance of fungal origin.Nature 171 344.
  • 10
    Doherty P., Furness J., Williams E.J., Walsh F.S. (1994) Neurite outgrowth stimulated by tyrosine kinase inhibitor herbimycin A requires activation of tyrosine kinases and protein kinase C.J. Neurochem. 62 21242131.
  • 11
    Dudek H., Datta S.R., Franke T.F., Bimbaum M.J., Yao R., Cooper G.M., Segal R.A., Kaplan D.R., Greenberg M.E. (1997) Regulation of neuronal survival by the serine-threonine protein kinase Akt.Science 275 661665.
  • 12
    Franklin J.L., Sanz-Rodriguez C., Juhasz A., Deckwerth T.L., Johnson E.M.J r. (1995) Chronic depolarization prevents programmed death of sympathetic neurons in vitro but does not support growth: requirement for Ca2+ influx but not Trk activation.J. Neurosci. 15 643664.
  • 13
    Garcia I., Martinou I., Tsujimoto Y., Martinou J. -C. (1992) Prevention of programmed cell death of sympathetic neurons by bcl-2 proto-oncogene.Science 258 302304.
  • 14
    Jackson T.R., Blader I.J., Hammonds-Odie L.P., Burga C.R., Cooke F., Hawkins P.T., Wolf A.G., Heldman K.A., Theibert A.B. (1996) Initiation and maintenance of NGF-stimulated neurite out-growth requires activation of phosphoinositide 3-kinase.J. Cell Sci. 109 289300.
  • 15
    Kennedy S.G., Wagner A.J., Conzen S.D., Jordán J., Bellacosa A., Tsichlis P.N., Hay H. (1997) The P13-kinase/Akt signaling pathway delivers an anti-apoptotic signal.Genes Dev. 11 3301833025.
  • 16
    Kimura K., Hattori S., Kabuyama Y., Shizawa Y., Takayanagi J., Nakamura S., Toki S., Matsuda Y., Onodera K., Fukui Y. (1994) Neurite outgrowth of PC12 cells is suppressed by wortmannin, a specific inhibitor of phosphatidylinositol 3-kinase.J. Biol. Chem. 269 1896118967.
  • 17
    Kita Y., Kimura K.D., Kobayashi M., Ihara S., Kaibuchi K., Kuroda S., Ui M., Iba H., Konishi H., Kikkawa U., Nagata S., Fukui Y. (1998) Microinjection of activated phosphatidylinositol-3 kinase induces process outgrowth in rat PC12 cells through the Rac-JNK signal transduction pathway.J. Cell Sci. 111 907915.
  • 18
    Knüsel B., Kaplan D.R., Winslow J.W., Rosenthal A., Burton L.E., Beck K.D., Rabin S., Nikolics K., Hefti F. (1992) K-252b selectively potentiates cellular actions and trk tyrosine phosphorylation mediated by neurotrophin-3.J. Neurochem. 59 715722.
  • 19
    Kobayashi M., Nagata S., Kita Y., Nakatsu N., Ihara S., Kaibuchi K., Kuroda S., Ui M., Iba H., Konishi H., Kikkawa U., Saitoh I., Fukui Y. (1997) Expression of a constitutively active phosphatidylinositol 3-kinase induces process formation in rat PC12 cells.J. Biol. Chem. 272 1608916092.
  • 20
    Kwon H.J., Yoshida M., Fukui Y., Horinouchi S., Beppu T. (1992) Potent and specific inhibition of p60v-src protein kinase both in vivo and in vitro by radicicol.Cancer Res. 52 69266930.
  • 21
    Kwon H.J., Yoshida M., Muroya K., Hattori S., Nishida E., Fukui Y., Beppu T., Horinouchi S. (1995) Morphology of ras-transformed cells becomes apparently normal again with tyrosine kinase inhibitors without a decrease in the Ras-GTP complex. J. Biochem. (Tokyo) 118 221228.
  • 22
    Maisonpierre P.C., Belluscio L., Squinto S., Ip N.Y., Furth M.E., Rinsay R.M., Yancopoulos G.D. (1990) Neurotrophin-3: a neurotrophic factor related to NGF and BDNF.Science 247 14461451.
  • 23
    Marshall C.J. (1995) Specificity of receptor tyrosine kinase signaling; transient versus sustained extracellular signal-regulated kinase activation.Cell 80 179185.
  • 24
    McCapra F., Scott A.I., Delmotte P., Delmotte-Plaquee J., Bhacca N.S. (1964) The constitution of monorden, an antibiotic with tranquilizing action.Tetrahedron Lett. 15 869875.
  • 25
    McMahon S.B., Armanini M.P., Lanway H.L., Phillips H.S. (1994) Expression and coexpression of Trk receptors in subpopulations of adult primary sensory neurons projecting to identified peripheral targets.Neurons 12 11611171.
  • 26
    Nobes C.D., Reppas J.B., Markus A., Tolkovsky A.M. (1996) Active p21 ras is sufficient for rescue of NGF-dependent rat sympathetic neurons.Neuroscience 70 10671079.
  • 27
    Qiu M. & Green S.H. (1992) PC12 cell neuronal differentiation is associated with prolonged p21ras activity and consequent prolonged ERK activity.Neuron 9 705717.
  • 28
    Rosenthal A., Goeddel D.V., Hguyen T., Lewis M., Shih A., Laramee G.R., Nikolics K., Winslow J.W. (1990) Primary structure and biological activity of a novel human neurotrophic factor.Neuron 4 767773.
  • 29
    Ruit K.G., Elliott J.L., Osborne P.A., Yan Q., Snider W.D. (1992) Selective dependence of mammalian dorsal root ganglion neurons on nerve growth factor during embryonic development.Neuron 8 573587.
  • 30
    Sano M. & Kitajima S. (1998) Activation of mitogen-activated protein kinases is not required for the extension of neurites from PC12D cells triggered by nerve growth factor.Brain Res. 785 299308.
  • 31
    Sano M., Iwanaga M., Fujisawa H., Nagahama M., Yamazaki Y. (1994) Staurosporine induces the outgrowth of neurites from the dorsal root ganglion of the chick embryo and PC12D cells.Brain Res. 639 115124.
  • 32
    Schmidt M.F. & Kater S.B. (1993) Fibroblast growth factors, depolarization, and substratum interact in a combinatorial way to promote neuronal survival.Dev. Biol. 158 228237.
  • 33
    Sharma S.V., Agatsuma T., Nakano H. (1998) Targeting of the protein chaperone, HSP90, by the transformation suppressing agent, radicicol.Oncogene 16 26392645.
  • 34
    Soga S., Kozawa T., Narumi H., Akinaga S., Irie K., Matsumoto K., Sharma S.V., Nakano H., Mizukami T., Hara M. (1998) Radicicol leads to selective depletion of Raf kinase and disrupts K-Ras-activated aberrant signaling pathway.J. Biol. Chem. 273 822828.
  • 35
    Wright D.E. & Snider W.D. (1995) Neurotrophin receptor mRNA expression defines distinct populations of neurons in rat dorsal root ganglia.J. Comp. Neurol. 351 329338.
  • 36
    Xia Z., Dickens M., Raingeaud J., Davis R.J., Greenberg M.E. (1995) Opposing effects of ERK and JUK-p38 MAP kinases on apoptosis.Science 270 13261331.
  • 37
    Yan C.Y.I., Ferrari G., Greene L.A. (1995) N-Acetylcysteine-promoted survival of PC12 cells is glutathione-independent but transcription-dependent. J. Biol. Chem. 270 2682726832.
  • 38
    Yao R. & Cooper G.M. (1995) Requirement for phosphatidylinositol-3 kinase in the prevention of apoptosis by nerve growth factor.Science 267 20032005.
  • 39
    Yao R., Yoshihara M., Osada H. (1997) Specific activation of a c-Jun NH2-terminal kinase isoform and induction of neurite outgrowth in PC-12 cells by staurosporine. J. Biol. Chem. 272 1826118266.