The Salt-Inducible Kinase, SIK, Is Induced by Depolarization in Brain

Authors

  • Jonathan D. Feldman,

    1. Department of Pediatrics, University of Southern California, Los Angeles, California, U.S.A.*Department of Biological Chemistry, University of Southern California, Los Angeles, California, U.S.A.Department of Molecular and Medical Pharmacology, University of Southern California, Los Angeles, California, U.S.A.Molecular Biology Institute, UCLA Center for the Health Sciences, University of Southern California, Los Angeles, California, U.S.A. § Neuroscience Program, University of Southern California, Los Angeles, California, U.S.A.Department of General and Environmental Physiology, University of Naples “Federico II,” Naples, Italy
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  • Linda Vician,

    1. Department of Pediatrics, University of Southern California, Los Angeles, California, U.S.A.*Department of Biological Chemistry, University of Southern California, Los Angeles, California, U.S.A.Department of Molecular and Medical Pharmacology, University of Southern California, Los Angeles, California, U.S.A.Molecular Biology Institute, UCLA Center for the Health Sciences, University of Southern California, Los Angeles, California, U.S.A. § Neuroscience Program, University of Southern California, Los Angeles, California, U.S.A.Department of General and Environmental Physiology, University of Naples “Federico II,” Naples, Italy
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  • * Marianna Crispino,

    1. Department of Pediatrics, University of Southern California, Los Angeles, California, U.S.A.*Department of Biological Chemistry, University of Southern California, Los Angeles, California, U.S.A.Department of Molecular and Medical Pharmacology, University of Southern California, Los Angeles, California, U.S.A.Molecular Biology Institute, UCLA Center for the Health Sciences, University of Southern California, Los Angeles, California, U.S.A. § Neuroscience Program, University of Southern California, Los Angeles, California, U.S.A.Department of General and Environmental Physiology, University of Naples “Federico II,” Naples, Italy
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  • Warren Hoe,

    1. Department of Pediatrics, University of Southern California, Los Angeles, California, U.S.A.*Department of Biological Chemistry, University of Southern California, Los Angeles, California, U.S.A.Department of Molecular and Medical Pharmacology, University of Southern California, Los Angeles, California, U.S.A.Molecular Biology Institute, UCLA Center for the Health Sciences, University of Southern California, Los Angeles, California, U.S.A. § Neuroscience Program, University of Southern California, Los Angeles, California, U.S.A.Department of General and Environmental Physiology, University of Naples “Federico II,” Naples, Italy
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  • Michel Baudry,

    1. Department of Pediatrics, University of Southern California, Los Angeles, California, U.S.A.*Department of Biological Chemistry, University of Southern California, Los Angeles, California, U.S.A.Department of Molecular and Medical Pharmacology, University of Southern California, Los Angeles, California, U.S.A.Molecular Biology Institute, UCLA Center for the Health Sciences, University of Southern California, Los Angeles, California, U.S.A. § Neuroscience Program, University of Southern California, Los Angeles, California, U.S.A.Department of General and Environmental Physiology, University of Naples “Federico II,” Naples, Italy
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  • and * Harvey R. Herschman

    1. Department of Pediatrics, University of Southern California, Los Angeles, California, U.S.A.*Department of Biological Chemistry, University of Southern California, Los Angeles, California, U.S.A.Department of Molecular and Medical Pharmacology, University of Southern California, Los Angeles, California, U.S.A.Molecular Biology Institute, UCLA Center for the Health Sciences, University of Southern California, Los Angeles, California, U.S.A. § Neuroscience Program, University of Southern California, Los Angeles, California, U.S.A.Department of General and Environmental Physiology, University of Naples “Federico II,” Naples, Italy
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  • Lippincott Williams & Wilkins, Inc., Philadelphia

  • Abbreviations used: AMPK, AMP-activated protein kinase; CHX, cycloheximide; CPG, candidate plasticity-related gene; CREB, cyclic AMP-responsive element-binding protein; EGF, epidermal growth factor; GST, glutathione S-transferase; IEG, immediate-early gene; IPTG, isopropyl 1-thio-β-D-galactoside; KA, kainic acid; KID-2, kinase induced by depolarization-2; msk, myocardial sucrose-nonfermenting 1 protein kinase-like kinase; NGF, nerve growth factor; PRMT3, protein-arginine N-methyltransferase 3; RDA, representational difference analysis; SIK, salt-inducible kinase; SIKKD, salt-inducible kinase kinase domain; SNF1, sucrose-nonfermenting 1 protein kinase. The nucleotide sequences discussed in this article are deposited in the GenBank database under the following GenBank accession numbers: KID-2, AF106937 (present study); SIK, AB020480 (Wang et al., 1999); and msk, U11494 (Ruiz et al., 1994).

Address correspondence and reprint requests to Dr. H. R. Herschman at Molecular Biology Institute, UCLA Center for the Health Sciences, 611 Charles E. Young Drive East, Los Angeles, CA 90095-1570, U.S.A. E-mail: hherschman@mednet.ucla.edu

Abstract

Abstract: Membrane depolarization of neurons is thought to lead to changes in gene expression that modulate neuronal plasticity. We used representational difference analysis to identify a group of cDNAs that are induced by membrane depolarization or by forskolin, but not by neurotrophins or growth factors, in PC12 pheochromocytoma cells. One of these genes, SIK (salt-inducible kinase), is a member of the sucrose-nonfermenting 1 protein kinase/AMP-activated protein kinase protein kinase family that was also recently identified from the adrenal gland of rats treated with high-salt diets. SIK mRNA is induced up to eightfold in specific regions of the hippocampus and cortex in rats, following systemic kainic acid administration and seizure induction.

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