The current address of Dr. I. Lengyel is Institute of Biochemistry, Biological Research Centre, Szeged, Hungary.
Modulation of the Phosphorylation and Activity of Calcium/Calmodulin-Dependent Protein Kinase II by Zinc
Article first published online: 4 JAN 2002
Journal of Neurochemistry
Volume 75, Issue 2, pages 594–605, August 2000
How to Cite
Lengyel, I., Fieuw-Makaroff, S., Hall, A. L., Sim, A. T. R., Rostas, J. A. P. and Dunkley, P. R. (2000), Modulation of the Phosphorylation and Activity of Calcium/Calmodulin-Dependent Protein Kinase II by Zinc. Journal of Neurochemistry, 75: 594–605. doi: 10.1046/j.1471-4159.2000.0750594.x
Abbreviations used : BCIP, 5-bromo-4-chloro-3-indolyl phosphate p-toluidine ; CaM, calmodulin ; CaMPK-II, calcium/calmodulin-dependent protein kinase II ; NBT, nitro blue tetrazolium ; PAGE, polyacrylamide gel electrophoresis ; SDS, sodium dodecyl sulfate ; TBS, Trisbuffered saline ; TBST, TBS with Tween 100.
- Issue published online: 4 JAN 2002
- Article first published online: 4 JAN 2002
- Calcium/calmodulin-stimulated protein kinase II;
- Autonomous activity;
- Phosphorylation site analysis
Calcium/calmodulin-dependent protein kinase II (CaMPK-II) is a key regulatory enzyme in living cells. Modulation of its activity, therefore, could have a major impact on many cellular processes. We found that Zn2+ has multiple functional effects on CaMPK-II. Zn2+ generated a Ca2+/CaM-independent activity that correlated with the autophosphorylation of Thr286, inhibited Ca2+/CaM binding that correlated with the autophosphorylation of Thr306, and inhibited CaMPK-II activity at high concentrations that correlated with the autophosphorylation of Ser279. The relative level of autophosphorylation of these three sites was dependent on the concentration of zinc used. The autophosphorylation of at least these three sites, together with Zn2+ binding, generated an increased mobility form of CaMPK-II on sodium dodecyl sulfate gels. Overall, autophosphorylation induced by Zn2+ converts CaMPK-II into a different form than the binding of Ca2+/CaM. In certain nerve terminals, where Zn2+ has been shown to play a neuromodulatory role and is present in high concentrations, Zn2+ may turn CaMPK-II into a form that would be unable to respond to calcium signals.