Gαolf is necessary for coupling D1 and A2a receptors to adenylyl cyclase in the striatum
Article first published online: 20 DEC 2001
Journal of Neurochemistry
Volume 76, Issue 5, pages 1585–1588, March 2001
How to Cite
Corvol, J. C., Studler, J. M., Schonn, J. S., Girault, J. A. and Hervé, D. (2001), Gαolf is necessary for coupling D1 and A2a receptors to adenylyl cyclase in the striatum. Journal of Neurochemistry, 76: 1585–1588. doi: 10.1046/j.1471-4159.2001.00201.x
- Issue published online: 20 DEC 2001
- Article first published online: 20 DEC 2001
- Received October 12, 2000; revised manuscript received November 29, 2000; accepted December 4, 2000.
- A2a receptor;
- adenylyl cyclase;
- D1 receptor;
In the brain, dopamine and adenosine stimulate cyclic AMP (cAMP) production through D1 and A2a receptors, respectively. Using mutant mice deficient in the olfactory isoform of the stimulatory GTP-binding protein α subunit, Gαolf, we demonstrate here the obligatory role of this protein in the adenylyl cyclase responses to dopamine and adenosine in the caudate putamen. Responses to dopamine were also dramatically decreased in the nucleus accumbens but remained unaffected in the prefrontal cortex. Moreover, in the caudate putamen of mice heterozygous for the mutation, the amounts of Gαolf were half of the normal levels, and the efficacy of dopamine- and CGS 21680 A2 agonist-stimulated cAMP production was decreased. Together, these results identify Gαolf as a critical parameter in the responses to dopamine and adenosine in the basal ganglia.