Trafficking of green fluorescent protein tagged-vesicular acetylcholine transporter to varicosities in a cholinergic cell line



The publishers wish to apologize for the substandard printing of Figs 4 and 5 of the above article that appeared in J. Neurochem.78, pp. 1104–1113. We reproduce Figs 4 and 5 below as they should have appeared.

Figure 4.

 GFP–VAChT is present in early and recycling endosomes. SN56 cells were transfected with GFP–VAChT and examined by confocal microscopy (a and c). (b) Labeling of cells in (a) with Tfn-TxR for 5 min at 37°C. Arrows indicate some of the structures labeled by both GFP–VAChT and Tfn-TxR. Bar, 10 μm. (d) Labeling of cells in (c) with Tfn-TxR for 20 min at 37°C. Arrows indicate colocalization of GFP–VAChT and transferrin in recycling endosomes. The arrowhead shows colocalization in early endosomes. Bar, 20 μm.

Figure 5.

 GFP–VAChT is present in Rab5-positive endosomes. (a) Cells were transfected with GFP–Rab5 construct, and living cells were examined by serial Z-sectioning. The maximum Z projection is presented. (b) Image of the same cell as in (a) stained with FM4-64 for 30 min. Arrows indicate colocalization of GFP–Rab5 and FM4-64. (c) Coexpression of GFP–VAChT and the constitutively activated Rab5 mutant Q79L. Cells were examined by serial Z-sectioning and the maximum Z projection is shown. Expression of Rab5:Q79L results in larger endosomes visualized after incubation with FM4-64 (c2). GFP–VAChT (c1) also appears in structures larger than that observed normally (e.g. Figs 1, 2, 3 and 4), which have FM4-64 (c2). Bars, 20 μm.