The present address of Arjan Geerlings is Puleva Biotech, Camino de Purchil 66, 18004 Granada, Spain.
Glycine transporter isoforms show differential subcellular localization in PC12 cells
Article first published online: 25 JUN 2002
Journal of Neurochemistry
Volume 82, Issue 1, pages 58–65, July 2002
How to Cite
Geerlings, A., Núñez, E., Rodenstein, L., López-Corcuera, B. and Aragón, C. (2002), Glycine transporter isoforms show differential subcellular localization in PC12 cells. Journal of Neurochemistry, 82: 58–65. doi: 10.1046/j.1471-4159.2002.00930.x
- Issue published online: 25 JUN 2002
- Article first published online: 25 JUN 2002
- Received September 28, 2001; revised manuscript received March 7, 2002; accepted March 14, 2002.
- GFP fusion proteins;
- glycine re-uptake;
- neurotransmitter transporters;
- SNARE proteins;
- synaptic vesicle
The subcellular localization of glycine transporters one (GLYT1) and two (GLYT2) stably expressed in PC12 cells has been studied. To facilitate visualization, enhanced green fluorescent protein (GFP) was fused to the amino terminus of both glycine transporters. Functional analysis of the GFP–GLYT1 and GFP–GLYT2 stable cell lines demonstrated that they exhibited high affinity for glycine and the characteristic properties of both glycine transporter subtypes. The GFP-coupled transporters were differently distributed throughout the cell. GFP–GLYT1 was mainly localized on the plasma membrane, whereas most of GFP–GLYT2 was present on large dense-core vesicles and endosomes. Both transporters were absent from the synaptic vesicle population in PC12 cells.