Protective action of the peroxisome proliferator-activated receptor-γ agonist pioglitazone in a mouse model of Parkinson's disease
Article first published online: 24 JUL 2002
Journal of Neurochemistry
Volume 82, Issue 3, pages 615–624, August 2002
How to Cite
Breidert, T., Callebert, J., Heneka, M. T., Landreth, G., Launay, J. M. and Hirsch, E. C. (2002), Protective action of the peroxisome proliferator-activated receptor-γ agonist pioglitazone in a mouse model of Parkinson's disease. Journal of Neurochemistry, 82: 615–624. doi: 10.1046/j.1471-4159.2002.00990.x
- Issue published online: 24 JUL 2002
- Article first published online: 24 JUL 2002
- Received December 14, 2001; revised manuscript received April 16, 2002; accepted April 19, 2002.
- Parkinson's disease;
- peroxisome-proliferator-activated receptor-γ;
We examined the effect of pioglitazone, a peroxisome proliferator-activated receptor-γ (PPARγ)agonistofthethiazolidinedione class, on dopaminergic nerve cell death and glial activation in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson's disease. The acute intoxication of C57BL/6 mice with MPTP led to nigrostriatal injury, as determined by tyrosine hydroxylase (TH) immunocytochemistry, and HPLC detection of striatal dopamine and metabolites. Damage to the nigrostriatal dopamine system was accompanied by a transient activation of microglia, as determined by macrophage antigen-1 (Mac-1) and inducible nitric oxide synthase (iNOS) immunoreactivity, and a prolonged astrocytic response. Orally administered pioglitazone (∼ 20 mg/kg/day) attenuated the MPTP-inducedglialactivation and prevented the dopaminergic cell loss in the substantia nigra pars compacta (SNpc). In contrast, there was little reduction of MPTP-induced dopamine depletion, with no detectable effect on loss of TH immunoreactivity and glial response in the striatum of pioglitazone-treated animals. Low levels of PPARγ expression were detected in the ventral mesencephalon and striatum, and were unaffected by MPTP or pioglitazone treatment. Since pioglitazone affects primarily the SNpc in our model, different PPARγ-independent mechanisms may regulate glial activation in the dopaminergic terminals compared with the dopaminergic cell bodies after acute MPTP intoxication.