The binding of prion proteins to serum components is affected by detergent extraction conditions
Version of Record online: 25 JUN 2002
Journal of Neurochemistry
Volume 82, Issue 1, pages 1–5, July 2002
How to Cite
Shaked, Y., Engelstein, R. and Gabizon, R. (2002), The binding of prion proteins to serum components is affected by detergent extraction conditions. Journal of Neurochemistry, 82: 1–5. doi: 10.1046/j.1471-4159.2002.00995.x
- Issue online: 25 JUN 2002
- Version of Record online: 25 JUN 2002
- Received December 6, 2001; revised manuscript received December 31, 2001; accepted January 2, 2002.
- prion protein;
As many GPI anchored proteins, PrPC and its abnormal conformer PrPSc, are inserted into membrane microdomains known as rafts. Upon raft disruption, PrPC becomes soluble, while PrPSc aggregates into insoluble structures. It was recently published that, as opposed to PrPC, PrPSc, as well as its protease resistant core PrP27-30, can bind specifically to plasminogen and other serum components. These findings were suggested to have important physiological implications in transmissible spongiform encephalopathies (TSE) diagnosis and pathogenesis. In this work, we show that the binding of PrPSc or PrP 27–30 to serum proteins occurs only at specific detergent combinations, in which disease associated PrPs are present in aggregated structures. At detergent conditions in which rafts are intact, it is actually PrPC. that binds to blood proteins, albeit not directly, but through neighboring rafts components. Our results therefore indicate that the binding of PrPSc to blood components has no physiological relevance.