Erucylphosphocholine-induced apoptosis in glioma cells: involvement of death receptor signalling and caspase activation
Article first published online: 3 FEB 2004
Journal of Neurochemistry
Volume 82, Issue 5, pages 1160–1170, September 2002
How to Cite
Kugler, W., Erdlenbruch, B., Jünemann, A., Heinemann, D., Eibl, H. and Lakomek, M. (2002), Erucylphosphocholine-induced apoptosis in glioma cells: involvement of death receptor signalling and caspase activation. Journal of Neurochemistry, 82: 1160–1170. doi: 10.1046/j.1471-4159.2002.01034.x
- Issue published online: 3 FEB 2004
- Article first published online: 3 FEB 2004
- Received February 15, 2002; revised manuscript received April 29, 2002; accepted May 21, 2002.
- death receptor signalling;
Erucylphosphocholine (ErPC) is a promising anti-neoplastic drug for the treatment of malignant brain tumours. It exerts strong anti-cancer activity in vivo and in vitro and induces apoptosis even in chemoresistant glioma cell lines. The purpose of this study was to expand on our previous observations on the potential mechanisms of ErPC-mediated apoptosis with a focus on death receptor activation and the caspase network. A172 and T98G glioma cells were treated with ErPC for up to 48 h. ErPC effects on the expression of the tumour necrosis factor (TNF) and TNF-related apoptosis-inducing ligand (TRAIL) receptor system, and on caspase activation were determined. ErPC had no effect on the expression of TNFα or TRAIL. Inhibition of the TNF or TRAIL signalling pathway with antagonistic antibodies or fusion proteins did not affect apoptosis induced by ErPC, and a dominant-negative FADD construct did not abolish ErPC-induced effects. Western blot analysis indicated that ErPC-triggered apoptosis resulted in a time-dependent processing of caspases-3, -7, -8 and -9 into their respective active subunits. Co-treatment of A172 cells with different caspase inhibitors prevented apoptosis but did not abrogate cell death. These data suggest that A172 cells might have an additional caspase-independent pathway that insures cell death and guarantees killing of those tumour cells whose caspase pathway is incomplete.