You have full text access to this OnlineOpen article

Na+ stimulates binding of dopamine to the dopamine transporter in cells but not in cell-free preparations

  1. Nianhang Chen,
  2. Judy Rickey,
  3. Maarten E. A. Reith

Article first published online: 4 JUL 2003

DOI: 10.1046/j.1471-4159.2003.01889.x

Issue

Journal of Neurochemistry

Journal of Neurochemistry

Volume 86, Issue 3, pages 678–686, August 2003

Additional Information

How to Cite

Chen, N., Rickey, J. and Reith, M. E. A. (2003), Na+ stimulates binding of dopamine to the dopamine transporter in cells but not in cell-free preparations. Journal of Neurochemistry, 86: 678–686. doi: 10.1046/j.1471-4159.2003.01889.x

Author Information

  1. Department of Biomedical and Therapeutic Sciences, University of Illinois College of Medicine, Peoria, Illinois, USA

*Address correspondence and reprint requests to Nianhang Chen, MD, Department of Biomedical and Therapeutic Sciences, University of Illinois College of Medicine, Box 1649, Peoria, IL 61656, USA. E-mail: nhc@uic.edu

Publication History

  1. Issue published online: 4 JUL 2003
  2. Article first published online: 4 JUL 2003
  3. Received March 28, 2003; revised manuscript received April 25, 2003; accepted April 28, 2003.

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (333K)

References

  • Amara S. G. and Kuhar M. J. (1993) Neurotransmitter transporters: recent progress. Annu. Rev. Neurosci. 16, 73–93.
  • Amara S. G. and Sonders M. S. (1998) Neurotransmitter transporters as molecular targets for addictive drugs. Drug alcohol. Dependency 51, 87–96.
  • Amejdki-Chab N., Benmansour S., Costentin J. and Bonnet J.-J. (1992) Effects of several cations on the neuronal uptake of dopamine and the specific binding of [3H]GBR12783: attempts to characterize the Na+ dependence of the neuronal transport of dopamine. J. Neurochem. 59, 1795–1804.
    Direct Link:
  • Blakely R. D. and Bauman A. L. (2000) Biogenic amine transporters: regulation in flux. Curr. Opin. Neurobiol. 10, 328–336.
  • Chen N. and Reith M. E. A. (2002) Structure-function relationships for biogenic amine neurotransmitter transporters, in Neurotransmitter Transporter: Structure, Function, and Regulation (Reith, M. E. A., ed.), pp. 25–34. Humana Press Inc., Totowa, NJ.
  • Chen N., Trowbridge C. G. and Justice J. B. Jr (1999) Cationic modulation of human dopamine transporter: dopamine uptake and inhibition of uptake. J. Pharmacol. Exp. Ther. 290, 940–949.
  • Chen N., Ferrer J. V., Javitch J. A. and Justice J. B. Jr (2000) Transport-dependent accessibility of a cytoplasmic loop cysteine in the human dopamine transporter. J. Biol. Chem. 275, 1608–1614.
  • Chen N., Vaughan R. A. and Reith M. E. A. (2001) The role of conserved tryptophan and acidic residues in the human dopamine transporters as characterized by site-directed mutagenesis. J. Neurochem. 77, 1116–1127.
    Direct Link:
  • Chen N., Sun L. and Reith M. E. A. (2002) Cationic interactions at the human dopamine transporter reveal binding conformations for dopamine distinguishable from those for the cocaine analog 2β-carbomethoxy-3β-(4-fluorophenyl) tropane. J. Neurochem. 81, 1383–1393.
    Direct Link:
  • Corera A. T., Costentin J. and Bonnet J.-J. (2000) Binding of uptake blockers to the neuronal dopamine transporter: further investigation about cationic and anionic requirements. Naunyn-Schmiedeberg's Arch. Pharmacol. 362, 213–221.
  • Dall'Asta V., Gatti R., Drlandini G., Rossi P. A., Rotoli B. M., Sala R., Bussolati O. and Gazzola G. C. (1997) Membrane potential changes visualized in complete growth media through confocal scanning microscopy of bis-oxonal-loaded cells. Exp. Cell Res. 231, 260–268.
  • Gainetdinov R. R. and Caron M. G. (2002) Monoamine transporters: their role in maintaining neuronal homeostasis, in Neurotransmitter Transporter: Structure, Function, and Regulation (ReithM. E. A., ed.), pp. 171–192. Humana Press Inc., Totowa, NJ.
  • Gu H., Wall S. and Rudnick G. (1994) Stable expression of biogenic amine transporters reveals differences in inhibitor sensitivity, kinetics, and ion dependence. J. Biol. Chem. 269, 7124–7130.
  • Hille B. (1992) Ionic Channels of Excitable Membranes, pp. 1–57. 2nd edn. Sinauer, Sutherland, MA.
  • Khoshbouei H., Wang H., Lechleiter J. D., Javitch J. A. and Galli A. (2003) Amphetamine-induced DA efflux: a voltage sensitive and intracellular Na+-dependent mechanism. J. Biol. Chem. 278, 102070–102077.
  • Li L. B. and Reith M. E. A. (1999) Modeling of the interaction of Na+ and K+ with the binding of dopamine and [3H]WIN 35,428 to the human dopamine transporter. J. Neurochem. 72, 1095–1109.
    Direct Link:
  • Li L. B., Cui X. N. and Reith M. E. A. (2002) Is Na+ required for the binding of dopamine, ampheramine, tyramine, and octopamine to the human dopamine transporter? Naunyn-Schmiedeberg's Arch. Pharmacol. 365, 303–311.
  • McElvain J. S. and Schenk J. O. (1992) A multisubstrate mechanism of striatal dopamine uptake and its inhibition by cocaine. Biochem. Pharmacol. 43, 2189–2199.
  • Reith M. E. A. and Coffey L. L. (1993) Cationic and anionic requirement for the binding of 2β-carbomethoxy-3β-(1-fluorophenyl) [3H]tropane to the dopamine carrier. J. Neurochem. 61, 167–177.
    Direct Link:
  • Robinson M. B. (2002) Regulated trafficking of neurotransmitter transporter: common notes but different melodies. J. Neurochem. 80, 1–11.
    Direct Link:
  • Rudnick G. (2002) Mechanisms of biogenic amine, in Neurotransmitter Transporter: Structure, Function, and Regulation (ReithM. E. A., ed.), pp. 25–52. Humana Press Inc., Totowa, NJ.
  • Schwartz J. W., Blakely R. D. and DeFelice L. J. (2003) Binding and transport in norepinephrine transporters: real-time, spatially resolved analysis in single cells using a fluorescent substrate. J. Biol. Chem. 278, 9768–9777.
  • Sonders M. S., Zhu S. J., Zahniser N. R., Kavanaugh M. P. and Amara S. G. (1997) Multiple ionic conductances of the human dopamine transporter: the actions of dopamine and psychostimulants. J. Neurosci. 17, 960–974.
  • Wu Q., Coffey L. L. and Reith M. E. A. (1997) Cations affect [3H]mazindol and [3H]WIN35,428 binding to the human dopamine transporter in a similar fashion. J. Neurochem. 69, 1106–1118.
  • Zahniser N. R. and Doolen S. (2001) Chronic and acute regulation of Na+/Cl–-dependent neurotransmitter transporters: drugs, substrates, presynaptic receptors, and signaling systems. Pharmacol. Ther. 92, 21–55.
View Full Article (HTML) Get PDF (333K)