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Characterization of microsatellite loci for Trigona carbonaria, a stingless bee endemic to Australia

Authors


: Dr Catherine Green. Fax: + 61 2 9351 4771; E-mail: cathygreen@primus.com.au

Abstract

Microsatellite loci were isolated from Trigona carbonaria (family Apidae; tribe Meliponini), a stingless bee endemic to Australia, following the screening of a partial genomic library with several simple-repeat oligonucleotide probes. Polymerase chain reaction (PCR) primers were designed to DNA sequence flanking a selection of trinucleotide and dinucleotide repeat sequences within positive clones. Nine of 10 microsatellite loci were found to be polymorphic amongst 20 unrelated T. carbonaria workers, with observed heterozygosity estimates ranging from 0.168 to 0.800. For many of the primers, distinct polymorphic products were also amplified when used in reactions with DNA from a selection of other bee species.

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