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Universal RAGE–PCR primers to detect mitochondrial polymorphisms among Magnoliophyta

Authors

  • L. Wijnants,

    1. Laboratoire de Physiologie Cellulaire et Morphogenèse Végétales, Université des Sciences et Techniques de Lille, bâtiment SN2, 59655 Villeneuve d’Ascq cedex, France
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  • J. Vasseur,

    1. Laboratoire de Physiologie Cellulaire et Morphogenèse Végétales, Université des Sciences et Techniques de Lille, bâtiment SN2, 59655 Villeneuve d’Ascq cedex, France
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  • C. Rambaud

    Corresponding author
    1. Laboratoire de Physiologie Cellulaire et Morphogenèse Végétales, Université des Sciences et Techniques de Lille, bâtiment SN2, 59655 Villeneuve d’Ascq cedex, France
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Caroline Rambaud. Fax: (33) 320 33 72 44; E-mail: Caroline.Rambaud@univ-lille1.fr

Abstract

A rapid amplification of genomic ends–polymerase chain reaction (RAGE–PCR) based technique was developed to detect polymorphism in mitochondrial intergenic sequences to compare cytoplasmic male sterile and revertant chicory. Here we describe 84 new mitochondrial primers that were successfully tested with two Liliopsida and four Magnoliopsida species and were useful for exploration of the mitochondrial genome in analyses such as population genetics or phylogenetical studies among Magnoliophyta.

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