Microsatellite DNA markers for the ground beetle Carabus insulicola

Authors

  • Y. Takami,

    Corresponding author
    1. Department of Natural History, Graduate School of Science, Tokyo Metropolitan University, Tokyo 192–0397, Japan,
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    • Present address: Center for Ecological Research, Kyoto University, Shiga 520-2113, Japan. Fax: + 81 (0)77 549 8201; E-mail: takami@ecology.kyoto-u.ac.jp

  • S. Katada

    1. Department of Biology, Graduate School of Science, Tokyo Metropolitan University, Tokyo 192–0397, Japan
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    • Present address: Tropical Biosphere Research Center, University of the Ryukyus, Iriomote Station, Okinawa 907–1541, Japan.


Yasuoki Takami.

Abstract

Twelve microsatellite DNA loci were newly isolated from the ground beetle Carabus insulicola, endemic to Japan, for studying mating systems. Phage vector was used for establishing genomic DNA library, and positive clones were screened with 32P-labelled probes. Primer sequences and annealing temperature for PCR amplification were determined for each locus. Allelic polymorphism of each locus, number of alleles and observed and expected heterozygosities, were investigated based on 24 individuals. Similarity between observed and expected heterozygosities suggests random mating, and relatively low observed heterozygosity found in two loci may be due to the presence of null alleles.

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