Characterization of microsatellite loci in the endangered St. Vincent Parrot, Amazona guildingii

Authors

  • M. Russello,

    1. Center for Environmental Research and Conservation, Columbia University, New York, NY, USA,
    2. Wildlife Conservation Society, Science Resource Center, Bronx, NY, USA,
    3. American Museum of Natural History, Molecular Systematics Laboratory, New York, NY, USA
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  • D. Calcagnotto,

    1. American Museum of Natural History, Molecular Systematics Laboratory, New York, NY, USA
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  • R. DeSalle,

    1. Center for Environmental Research and Conservation, Columbia University, New York, NY, USA,
    2. Wildlife Conservation Society, Science Resource Center, Bronx, NY, USA,
    3. American Museum of Natural History, Molecular Systematics Laboratory, New York, NY, USA
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  • G. Amato

    1. Center for Environmental Research and Conservation, Columbia University, New York, NY, USA,
    2. Wildlife Conservation Society, Science Resource Center, Bronx, NY, USA,
    3. American Museum of Natural History, Molecular Systematics Laboratory, New York, NY, USA
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M. Russello, American Museum of Natural History, Molecular Systematics Laboratory, Central Park West at 79th Street, New York, NY, USA 10024. Fax: (212) 769 5277; E-mail: russello@amnh.org

Abstract

Microsatellite loci were isolated within the St Vincent parrot (Amazona guildingii), an endangered island endemic, from a GTn enriched genomic library. Nine polymorphic loci were characterized varying from two to nine alleles per locus. Seven loci exhibited levels of heterozygosity ranging from 39 to 100% within the 28 individuals genotyped. Furthermore, the primers were demonstrated to cross-amplify within four genera of Neotropical parrots, indicating the potential utility of these markers to further population-level studies and conservation efforts of New World Psittacines.

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