Genetic variation in two populations of the rough-skinned newt (Taricha granulosa) assessed using novel tetranucleotide microsatellite loci

Authors


Adam G. Jones. Fax: (541) 737–0501; E-mail: jonesa@bcc.orst.edu

Abstract

Seven novel tetranucleotide microsatellite loci were identified from a partial genomic DNA library, enriched for GATA-motif microsatellites, from the rough-skinned newt (Taricha granulosa). All loci were polymorphic, and one displayed a high frequency null allele. A related species, T. rivularis, displays strong site fidelity and detectable population structure over small spatial scales, so we assessed genetic variation in two samples of T. granulosa separated by 16 km. Distributions of allele frequencies differ significantly between our two sites, but small values of FST and RhoST suggest that the populations are linked by a large amount of gene flow.

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