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gemini: software for testing the effects of genotyping errors and multitubes approach for individual identification

Authors

  • Nathaniel Valière,

    Corresponding author
    1. Laboratoire de Biométrie et Biologie Evolutive, UMR CNRS 5558, Université Claude Bernard Lyon 1, 43, boulevard du 11 novembre 1918, F-69622 Villeurbanne Cedex, France,
      Nathaniel Valière. Fax: +33(0) 478 892 719; E-mail: valiere@biomserv.univ-lyon1.fr
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  • Pierre Berthier,

    1. Populationsgenetik, Zoologisches Institut, Universität Bern, Balzerstrasse 3, CH-3012 Bern, Switzerland
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  • Dominique Mouchiroud,

    1. Laboratoire de Biométrie et Biologie Evolutive, UMR CNRS 5558, Université Claude Bernard Lyon 1, 43, boulevard du 11 novembre 1918, F-69622 Villeurbanne Cedex, France,
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  • Dominique Pontier

    1. Laboratoire de Biométrie et Biologie Evolutive, UMR CNRS 5558, Université Claude Bernard Lyon 1, 43, boulevard du 11 novembre 1918, F-69622 Villeurbanne Cedex, France,
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Nathaniel Valière. Fax: +33(0) 478 892 719; E-mail: valiere@biomserv.univ-lyon1.fr

Abstract

Improvements in the determination of individual genotypes from samples with low DNA quantity and quality are of prime importance in molecular ecology and conservation for reliable genetic individual identification (molecular tagging using microsatllites loci). Thus, errors (e.g. allelic dropout and false allele) appearing during samples genotyping must be monitored and eliminated as far as possible. The multitubes approach is a very effective but a costly and time-consuming solution. In this paper, we present a simulation software that allows evaluation of the effect of genotyping errors on genetic identification of individuals and the effectiveness of a multitubes approach to correct these errors.

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