Isolation and characterization of microsatellites in a perennial Apiaceae, Eryngium alpinum L.

Authors

  • M. Gaudeul,

    Corresponding author
    1. Laboratoire de Biologie des Populations d’Altitude, UMR CNRS 5553, Université J. Fourier, BP53, F-38041 Grenoble Cedex 09, France,
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  • Y. Naciri-Graven,

    1. Laboratoire de Biologie des Populations d’Altitude, UMR CNRS 5553, Université J. Fourier, BP53, F-38041 Grenoble Cedex 09, France,
    2. Conservatoire et Jardin Botaniques, 1 chemin de l’Impératrice, Case Postale 60, CH-1292 Chambésy, Genève, Switzerland
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  • P. Gauthier,

    1. Laboratoire de Biologie des Populations d’Altitude, UMR CNRS 5553, Université J. Fourier, BP53, F-38041 Grenoble Cedex 09, France,
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  • F. Pompanon

    1. Laboratoire de Biologie des Populations d’Altitude, UMR CNRS 5553, Université J. Fourier, BP53, F-38041 Grenoble Cedex 09, France,
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Myriam Gaudeul. Fax: + 33 4 76 51 42 79; E-mail: myriam.gaudeul@ujf-grenoble.fr

Abstract

In order to investigate the genetic structure in an endangered Alpine plant (Eryngium alpinum L.), we developed microsatellites. Two different approaches were used: an enrichment protocol and the classical technique of hybridization on nylon membranes. We identified 25 loci, 13 of which revealed to be polymorphic. The polymorphism was rather low (2–6 alleles; HE = 0.49 ± 0.16), probably due to the short size of microsatellites (6–10 dinucleotide repeats) and to the fine spatial scale investigated. However, these markers are expected to provide a new insight about the genetic processes at work within and among E. alpinum populations.

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