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Keywords:

  • Dendrocopos leucotos;
  • D. major;
  • Dryocopus martius;
  • mtDNA;
  • Picus awokera;
  • 12S rRNA

Abstract

In birds, feathers and faeces can be used as a source of DNA for genetic analyses. If the materials are derived from an absentee bird(s), however, the species must first be identified. Here, we developed four pairs of primers for amplification of mitochondrial 12S ribosomal RNA fragments in the black woodpecker Dryocopus martius, a vulnerable species in Japan, and three other sympatric woodpeckers including Dendrocopos leucotos, D. major and Picus awokera. Because each primer pair gave a PCR product in only one of the examined species, these primer sets will be useful in identifying the species of woodpeckers.