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Novel primer sets for species-specific amplification of the mitochondrial 12S rRNA genes in four Japanese woodpeckers (Picidae, Piciformes)

Authors

  • Mitsuhiro Nakamura,

    Corresponding author
    1. Tohoku Research Center, Forestry and Forest Products Research Institute, Morioka 020–0123, Japan,
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  • Yasuyuki Ishibashi,

    1. Hokkaido Research Center, Forestry and Forest Products Research Institute, Sapporo 062–8516, Japan,
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  • Abe Syuiti

    1. Laboratory of Animal Cytogenetics, Center for Advanced Science and Technology,
    2. Laboratory of Cytogenetics, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060–0810, Japan
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Mitsuhiro Nakamura. Fax: + 81 19 6416747; E-mail: nakmitu@ffpri.affrc.go.jp

Abstract

In birds, feathers and faeces can be used as a source of DNA for genetic analyses. If the materials are derived from an absentee bird(s), however, the species must first be identified. Here, we developed four pairs of primers for amplification of mitochondrial 12S ribosomal RNA fragments in the black woodpecker Dryocopus martius, a vulnerable species in Japan, and three other sympatric woodpeckers including Dendrocopos leucotos, D. major and Picus awokera. Because each primer pair gave a PCR product in only one of the examined species, these primer sets will be useful in identifying the species of woodpeckers.

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