Microsatellite markers for the Mexican spotted owl (Strix occidentalis lucida)


Jonathan L. Longmire. E-mail: longmire@telomere.lanl.gov


A genomic cosmid library was used to develop seven highly polymorphic microsatellite markers for the Mexican spotted owl (Strix occidentalis lucida). These are the first reported microsatellite markers derived from this species. The cloned and sequenced repeat motifs include a triplet repeat of (AAT)n, two tetranucleotide repeats of (GATA)n, a tetranucleotide repeat of (ATCC)n, a compound repeat of (GA)n(GATA)n and the two pentanucleotide repeats (AGAAT)n and (ATTTT)n. The microsatellites described represent six presumably independent loci with the two pentanucleotide repeats having originated from a single cosmid. Primer pairs allow locus-specific amplification of each marker from Mexican spotted owl genomic DNA.